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  • 1
    Language: English
    In: Blood, 2004, Vol.104 (12), p.3565-3572
    Description: The AML1/CBFbeta transcriptional complex is essential for the formation of definitive hematopoietic stem cells (HSCs). Moreover, development of the hematopoietic system is exquisitely sensitive to the level of this complex. To investigate the effect of AML1 dosage on adult hematopoiesis, we compared the hematopoietic systems of AML1+/- and AML1+/+ mice. Surprisingly, loss of a single AML1 allele resulted in a 50% reduction in long-term repopulating hematopoietic stem cells (LTR-HSCs). This decrease did not, however, extend to the next level of hematopoietic differentiation. Instead, AML1+/- mice had an increase in multilineage progenitors, an expansion that resulted in enhanced engraftment following transplantation. The expanded pool of AML1+/- progenitors remained responsive to homeostatic mechanisms and thus the number of mature cells in most lineages remained within normal limits. Two notable exceptions were a decrease in CD4(+) T cells, leading to an inversion of the CD4(+) to CD8(+) T-cell ratio and a decrease in circulating platelets. These data demonstrate a dosage-dependent role for AML1/CBFbeta in regulating the quantity of HSCs and their downstream committed progenitors, as well as a more restricted role in T cells and platelets. The latter defect mimics one of the key abnormalities in human patients with the familial platelet disorder resulting from AML1 haploinsufficiency.
    Subject(s): Fundamental and applied biological sciences. Psychology ; Biological and medical sciences ; Cell physiology ; Molecular and cellular biology ; Cell differentiation, maturation, development, hematopoiesis ; Haplotypes ; Proto-Oncogene Proteins - pharmacology ; DNA-Binding Proteins - pharmacology ; Transcription Factors - deficiency ; Cells, Cultured ; Graft Survival ; Proto-Oncogene Proteins - deficiency ; DNA-Binding Proteins - deficiency ; Blood Cell Count ; Mice, Knockout ; Dose-Response Relationship, Drug ; Cell Lineage ; Animals ; Hematopoiesis ; Transcription Factors - pharmacology ; Hematopoietic Stem Cells - cytology ; Bone Marrow Transplantation ; Core Binding Factor Alpha 2 Subunit ; Cell Differentiation ; Mice ; Cell Culture Techniques ; Index Medicus ; Abridged Index Medicus
    ISSN: 0006-4971
    E-ISSN: 1528-0020
    Source: HighWire Press (Free Journals)
    Source: Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
    Source: American Society of Hematology
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 2
    Language: English
    In: Cell structure and function, 2019, Vol.44 (1), p.29-39
    Description: PUM2, an RNA binding protein, is known to promote stem cell proliferation via repressing expressions of cell cycle genes. Similar with stem cells, malignant cells are characterized by unlimited proliferation and remote migration. However, roles of PUM2 in cancer development are controversial. Here, we investigated PUM2’s role in glioblastoma development and its relationship with the cell cycle regulator BTG1. Immunoblotting and RT-qPCR were used to evaluate protein expression level and transcript level, respectively. ShRNAs were designed to knock down PUM2 and BTG1 expression. CCK-8 assay was used to evaluate cell viability. Cell migration assay and evasion assay were used to evaluate metastatic capability of glioblastoma cell. RNA pull-down assay and RNA immunoprecipitation assay were used to test the interaction between PUM2 and BTG1 3’UTR. PUM2 expression is elevated in glioblastoma tumor tissues as well as glioblastoma cell lines. PUM2 knockdown remarkably suppresses glioblastoma cell proliferation and migration. In addition, PUM2 knockdown increases BTG1 expression. RNA pull-down assay and RNA immunoprecipitation assay show PUM2 binds to BTG1 3’UTR directly. Furthermore, knockdown of BTG1 reverses the effect of PUM2 knockdown on glioblastoma cell proliferation and migration. Our results suggest that PUM2 promote glioblastoma development via repressing BTG1 expression.Key words: PUM2, BTG1, glioblastoma, cell proliferation, metastasis
    Subject(s): BTG1 ; glioblastoma ; cell proliferation ; metastasis ; PUM2
    ISSN: 0386-7196
    E-ISSN: 1347-3700
    Source: Directory of Open Access Journals
    Source: ProQuest Central
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 3
    Language: English
    In: Science (American Association for the Advancement of Science), 2012-06-22, Vol.336 (6088), p.1585-1588
    Description: The formation of healthy gametes depends on programmed DNA double-strand breaks (DSBs), which are each repaired as a crossover (CO) or non-crossover (NCO) from a homologous template. Although most of these DSBs are repaired without giving COs, little is known about the genetic requirements of NCO-specific recombination. We show that Fml1, the Fanconi anemia complementation group M (FANCM)-ortholog of Schizosaccharomyces pombe, directs the formation of NCOs during meiosis in competition with the Mus81-dependent pro-CO pathway. We also define the Rad51/Dmc1-mediator Swi5-Sfr1 as a major determinant in biasing the recombination process in favor of Mus81, to ensure the appropriate amount of COs to guide meiotic chromosome segregation. The conservation of these proteins from yeast to humans suggests that this interplay may be a general feature of meiotic recombination.
    Subject(s): Yeasts ; Molecules ; DNA ; REPORTS ; Alleles ; Meiosis ; Viability ; Chromosome segregation ; Chromosomes ; Spores ; Crossovers ; Fundamental and applied biological sciences. Psychology ; Biological and medical sciences ; Cell physiology ; Molecular and cellular biology ; Cell cycle, cell proliferation ; Chromosome Segregation ; Endonucleases - genetics ; Recombinases - metabolism ; Crossing Over, Genetic ; Schizosaccharomyces pombe Proteins - genetics ; Recombinases - genetics ; Schizosaccharomyces - physiology ; Homologous Recombination ; DNA, Fungal - chemistry ; Endonucleases - metabolism ; DNA-Binding Proteins - genetics ; DNA Breaks, Double-Stranded ; DNA-Binding Proteins - metabolism ; Schizosaccharomyces - genetics ; DNA Helicases - metabolism ; DNA Repair ; DNA, Fungal - metabolism ; Schizosaccharomyces pombe Proteins - metabolism ; Mutation ; DNA Helicases - genetics ; Chromosomes, Fungal - physiology ; Yeast fungi ; Physiological aspects ; Development and progression ; Research ; Health aspects ; Recombinant proteins ; Fanconi's anemia ; Index Medicus ; Schizosaccharomyces pombe ; Fml1 ; Homologous recombination ; Mus81
    ISSN: 0036-8075
    E-ISSN: 1095-9203
    Source: Single Journals
    Source: Academic Search Ultimate
    Source: EBSCOhost EJS
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 4
    Language: English
    In: Journal of pediatric hematology/oncology, 2020-05, Vol.42 (4), p.261-265
    Description: Primary objective is to evaluate safety of isavuconazonium sulfate (ISA) in pediatrics below 18 years old. Exploratory endpoint includes mortality due to probable and proven invasive fungal infection (IFI) and overall morality in this population. Retrospective review of patients below 18 years receiving ISA for ≥7 days for possible, probable, or proven IFI or prophylaxis between June 2015 and March 2018. Descriptive analysis performed to calculate median, frequency, and percentages. Safety analysis included 18 patients and a subgroup of 11/18 patients were assessed for efficacy. Median age 12.5 years (4 to 17 y), median weight 50.25 kg (19 to 118 kg), 50% male, 77% acute leukemias, 94% hematopoietic cell transplant recipients, 50% matched unrelated donors and 78% in remission. Elevated alanine aminotransferase 3 times baseline within 30 days of ISA occurred in 22% (4/18). No patients had elevated bilirubin or increase in serum creatinine. All-cause mortality at 90 days was 22% (4/18) and 27% (3/11) in patients with probable or proven IFI. Clinical response rates: 14-day: 45% (5/11) partial, 27% (3/11) stable; 30-day: 45% (5/11) partial, 36% (4/11) stable; 90-day: 54% (6/11) had either partial (n=3) or complete (n=3) response to ISA. ISA is safe in pediatric patients for the treatment of IFI. Prospective, randomized controlled trials are warranted to determine efficacy and safety of ISA in pediatric patients with hematologic malignancies and hematopoietic cell transplant.
    Subject(s): Hematologic Neoplasms - therapy ; Triazoles - administration & dosage ; Triazoles - adverse effects ; Pyridines - administration & dosage ; Humans ; Child, Preschool ; Hematopoietic Stem Cell Transplantation ; Male ; Invasive Fungal Infections - blood ; Nitriles - administration & dosage ; Pyridines - adverse effects ; Hematologic Neoplasms - blood ; Invasive Fungal Infections - drug therapy ; Adolescent ; Invasive Fungal Infections - etiology ; Female ; Retrospective Studies ; Nitriles - adverse effects ; Child ; Index Medicus
    ISSN: 1077-4114
    E-ISSN: 1536-3678
    Source: Hellenic Academic Libraries Link
    Source: EBSCOhost EJS
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 5
    Language: English
    In: IEEE access, 2020, Vol.8, p.7916-7924
    Description: The timely and accurate detection of traffic incidents is beneficial to reduce associated economic losses and avoid secondary crashes. Inspired by the impressive success of the image classification algorithms, especially convolutional neural networks (CNNs), this study proposes a novel framework to detect highway traffic incidents by learning the traffic state as images. In such a framework, the probe vehicles equipped with the global positioning system equipment are used to obtain data. The Gramian Angular Difference Fields and Piecewise Aggregation Approximation algorithms are used to convert the link speed time series data into images. CNNs can extract the traffic features based on these images and consider an incident detection problem as a binary classification task. Further, the effectiveness of the proposed framework is evaluated by applying it to detect the traffic in a real-world environment, i.e., the Guangzhou Airport Expressway. The results illustrate that the proposed model outperforms several other algorithms with respect to almost all the performance indexes, including the detection rate with different false alarm rates and the area under the receiver operating characteristic curve.
    Subject(s): Gramian Angular Difference Fields ; Roads ; Time series analysis ; Detectors ; Predictive models ; Prediction algorithms ; Airports ; Convolutional neural networks ; incident detection ; Binary classification ; convolutional neural networks
    ISSN: 2169-3536
    E-ISSN: 2169-3536
    Source: Directory of Open Access Journals
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  • 6
    Language: English
    In: Leukemia, 2018-11, Vol.32 (11), p.2316-2325
    Description: The survival of pediatric patients with multiply relapsed and/or refractory (R/R) B-cell acute lymphoblastic leukemia has historically been very poor; however, data are limited in the current era. We conducted a retrospective study to determine the outcome of multiply R/R childhood B-ALL treated at 24 TACL institutions between 2005 and 2013. Patient information, treatment, and response were collected. Prognostic factors influencing the complete remission (CR) rate and event-free survival (EFS) were analyzed. The analytic set included 578 salvage treatment attempts among 325 patients. CR rates (mean ± SE) were 51 ± 4% for patients with bone marrow R/R B-ALL who underwent a second salvage attempt, 37 ± 6% for a third attempt, and 31 ± 6% for the fourth through eighth attempts combined. For patients achieving a CR after their second, third, and fourth through eighth attempts, the 2 year EFS was 41 ± 6%, 13 ± 7%, and 27 ± 13% respectively. Our results showed slight improvement when compared to previous studies. This is the largest and most recent study to date that evaluates the outcome of this patient population. Our data will provide detailed reference for the evaluation of new agents being developed for childhood B-ALL.
    ISSN: 0887-6924
    E-ISSN: 1476-5551
    Source: Nature Open Access
    Source: Alma/SFX Local Collection
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 7
    Language: English
    In: Journal of hematology and oncology, 2018-05-02, Vol.11 (1), p.61-5
    Description: Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a highly aggressive hematological malignancy with extremely poor outcome. The median overall survival for adult patients is 9-13 months. Pediatric patients are exceedingly rare with an unclear clinical course. Currently, no standardized therapy has been established, although an acute lymphoblastic leukemia type of treatment appears to be more effective in those patients who are able to tolerate aggressive chemotherapy. SL-401 is a targeted therapy directed to CD123, a protein ubiquitously expressed at high level on the surface of BPDCN blasts. In adult phase 2 trials, it has demonstrated efficacy with 90% overall response rate. No pediatric patients with BPDCN using SL-401 have been reported. Here, we report the first pediatric experience of three children with BPDCN treated with SL-401 at our institution. All patients tolerated SL-401 without significant toxicities. One patient with multiply relapsed and refractory disease had no response. The other two cases had significant and rapid clinical improvement after the two courses of treatment. However, the response was transient, and growth of soft tissue mass was observed in-between cycles in both patients with large tumor burden. This is the first report of SL-401 in pediatric patients with BPDCN. Sl-401 was well tolerated and can produce a promising response. Further testing this agent in children is warranted.
    Subject(s): Physiological aspects ; Care and treatment ; Lymphomas ; Genetic aspects ; Research ; Dendritic cells ; Targeted therapy ; Pediatric ; BPDCN ; SL-401 ; Case Report
    ISSN: 1756-8722
    E-ISSN: 1756-8722
    Source: BioMedCentral Open Access
    Source: Academic Search Ultimate
    Source: PubMed Central
    Source: Directory of Open Access Journals
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  • 8
    Language: English
    In: Current microbiology, 2017-10, Vol.74 (10), p.1169-1177
    Description: It is well documented that the microbial interactions and biodiversity play an important role in health and disease in mammalian species. There is a rare study about gut microbiota of Mustelidae family. In this study, 40 male and female minks from Northeast China were divided into three groups and fed until they reached maturity. The V3 region of 16S rRNA genes was amplified and sequenced using NGS. There were 526 OTUs principally concentrated among five bacterial phyla. Two points about mink's body weight gaining were observed: (1) the weight of male individuals increased more rapidly than female individuals; (2) the weight of individuals whose feed was supplemented with Chinese herb additives increased more rapidly than individuals without giving food additives. The differences of microorganism abundance were shown in two points: (1) two genera which had ≥2-fold change difference were found between male and female minks. (2) Ten genera which had a ≥2-fold change difference were found among minks with and without Chinese herb additive. Findings from this study provide new and fundamental knowledge on the gut microbiota composition of minks farmed in Northeast China, which can contribute to the general well-being of minks worldwide.
    Subject(s): Microbiota (Symbiotic organisms) ; RNA ; Molecular biology ; Food additives ; Body weight ; Index Medicus
    ISSN: 0343-8651
    E-ISSN: 1432-0991
    Source: Alma/SFX Local Collection
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 9
    Conference Proceeding
    Conference Proceeding
    2020
    ISSN: 2101-6275 
    Language: English
    In: EPJ Web of Conferences, 2020-01-01, Vol.239, p.03018
    Description: The deuteron elastic and inelastic scattering on 56Fe is calculated by using the coupled-channel method and the collective motion models. Based on the experimental angular distributions of scattering processes, an optimal set of parameters are obtained for the deuteron's coupled-channel optical model potential and 56Fe's deformation including first ten low-lying excited states. The calculation results are in good agreement with the experimental data at low incident energies (Ed 〈 30 MeV), except for 41+(2.085 MeV) and 23+(2.960 MeV), and the deviation is found for several states at 56 MeV. That indicates further analyses and discussions are necessary.
    Subject(s): Iron isotopes ; Deuterons ; Inelastic scattering ; Elastic scattering
    ISSN: 2101-6275
    E-ISSN: 2100-014X
    Source: Academic Search Ultimate
    Source: Directory of Open Access Journals
    Source: ProQuest Central
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 10
    Conference Proceeding
    Conference Proceeding
    2020
    ISSN: 2101-6275 
    Language: English
    In: EPJ Web of Conferences, 2020-01-01, Vol.239, p.14004
    Description: Ab initio molecular dynamics (AIMD) methods were used to calculate the density of states (DOS) for liquid molten salt Li2BeF4 (LiF-BeF2) in different temperatures. The Egelstaff and Schofield effective width model was used to obtain the diffusion-type spectrum of the DOS and the corresponding partial s (α, β). Finally, the thermal neutron scattering data for liquid molten salt LiF-BeF2 were given.
    Subject(s): Density of states ; Thermal neutrons ; Neutron scattering ; Lithium fluoride ; Molten salts ; Neutrons ; Molecular dynamics
    ISSN: 2101-6275
    E-ISSN: 2100-014X
    Source: Academic Search Ultimate
    Source: Directory of Open Access Journals
    Source: ProQuest Central
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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