placeholder
and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Proceed order?

Export
Filter
Document type
Language
Year
  • 1
    Language: English
    In: Proceedings of the National Academy of Sciences of the United States of America, 14 June 2016, Vol.113(24), pp.E3403-12
    Description: Type I IFNs are key cytokines mediating innate antiviral immunity. cGMP-AMP synthase, ritinoic acid-inducible protein 1 (RIG-I)-like receptors, and Toll-like receptors recognize microbial double-stranded (ds)DNA, dsRNA, and LPS to induce the expression of type I IFNs. These signaling pathways converge at the recruitment and activation of the transcription factor IRF-3 (IFN regulatory factor 3). The adaptor proteins STING (stimulator of IFN genes), MAVS (mitochondrial antiviral signaling), and TRIF (TIR domain-containing adaptor inducing IFN-β) mediate the recruitment of IRF-3 through a conserved pLxIS motif. Here we show that the pLxIS motif of phosphorylated STING, MAVS, and TRIF binds to IRF-3 in a similar manner, whereas residues upstream of the motif confer specificity. The structure of the IRF-3 phosphomimetic mutant S386/396E bound to the cAMP response element binding protein (CREB)-binding protein reveals that the pLxIS motif also mediates IRF-3 dimerization and activation. Moreover,...
    Subject(s): Crystal Structure ; Innate Immunity ; Signaling ; Transcription Factor ; Type I Interferon ; Interferon Regulatory Factor-3 -- Chemistry ; Rotavirus -- Chemistry ; Viral Nonstructural Proteins -- Chemistry
    ISSN: 0027-8424
    E-ISSN: 1091-6490
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Language: English
    In: The Biochemical journal, 01 February 2017, Vol.474(3), pp.427-443
    Description: Staphylococcus epidermidis is an opportunistic pathogen that can form robust biofilms that render the bacteria resistant to antibiotic action and immune responses. Intercellular adhesion in S. epidermidis biofilms is mediated by the cell wall-associated accumulation-associated protein (Aap), via zinc-mediated self-assembly of its B-repeat region. This region contains up to 17 nearly identical sequence repeats, with each repeat assumed to be functionally equivalent. However, Aap B-repeats exist as two subtypes, defined by a cluster of consensus or variant amino acids. These variable residues are positioned near the zinc-binding (and dimerization) site and the stability determinant for the B-repeat fold. We have characterized four B-repeat constructs to assess the functional relevance of the two Aap B-repeat subtypes. Analytical ultracentrifugation experiments demonstrated that constructs with the variant sequence show reduced or absent Zn-induced dimerization. Likewise, circular dichroism...
    Subject(s): Aap ; Staphylococcus ; Biofilm ; Dimerization ; Intercellular Adhesion ; Protein Stability ; Genetic Variation ; Bacterial Adhesion -- Physiology ; Bacterial Proteins -- Chemistry ; Biofilms -- Growth & Development ; Staphylococcus Epidermidis -- Physiology ; Zinc -- Chemistry
    ISSN: 0264-6021
    E-ISSN: 1470-8728
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Language: English
    In: Antimicrobial Agents and Chemotherapy, 10/2017, Vol.61(10)
    Description: Oxazolidinones are promising candidates for the treatment of Mycobacterium tuberculosis infections. We isolated linezolid-resistant strains from H37Rv (Euro-American) and HN878 (East-Asian) strains; resistance frequencies were similar in the two strains. Mutations were identified in ribosomal protein L3 (RplC) and the 23S rRNA (rrl). All mutant strains were cross resistant to sutezolid; a subset was cross resistant to chloramphenicol. Mutations in rrl led to growth impairment and decreased fitness that may limit spread in clinical settings.
    Subject(s): Medicine ; Biology ; Pharmacy, Therapeutics, & Pharmacology;
    ISSN: 0066-4804
    E-ISSN: 1098-6596
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Language: English
    In: PLoS Pathogens, 2011, Vol.7(11), p.e1002360
    Description: Antimicrobial peptides (AMPs) contribute to host innate immune defense and are a critical component to control bacterial infection. Nontypeable Haemophilus influenzae (NTHI) is a commensal inhabitant of the human nasopharyngeal mucosa, yet is commonly associated with opportunistic infections of the upper and lower respiratory tracts. An important aspect of NTHI virulence is the ability to avert bactericidal effects of host-derived antimicrobial peptides (AMPs). The Sap ( s ensitivity to a ntimicrobial p eptides) ABC transporter equips NTHI to resist AMPs, although the mechanism of this resistance has remained undefined. We previously determined that the periplasmic binding protein SapA bound AMPs and was required for NTHI virulence in vivo. We now demonstrate, by antibody-mediated neutralization of AMP in vivo, that SapA functions to directly counter AMP lethality during NTHI infection. We hypothesized that SapA would deliver AMPs to the Sap inner membrane complex for transport into the bacterial cytoplasm. We observed that AMPs localize to the bacterial cytoplasm of the parental NTHI strain and were susceptible to cytoplasmic peptidase activity. In striking contrast, AMPs accumulated in the periplasm of bacteria lacking a functional Sap permease complex. These data support a mechanism of Sap mediated import of AMPs, a novel strategy to reduce periplasmic and inner membrane accumulation of these host defense peptides. ; The opportunistic pathogen is a normal inhabitant of the human nasopharynx, and is commonly implicated in respiratory tract infections, particularly of the middle ear (otitis media), sinuses, and lung (pneumonia, chronic obstructive pulmonary disease and cystic fibrosis). We have identified a multifunctional bacterial uptake system that is required for critical mechanisms of bacterial survival in the host. This Sap transporter system recognizes and transports host immune defense molecules and is involved in uptake of an iron-containing nutrient (heme) that is host-limited, yet required for bacterial growth and survival. We propose that bacteria utilize this, and likely other similar transport systems, for numerous functions that are important for bacterial survival in the host, including host immune evasion and metabolism. Our findings significantly advance our understanding of how single bacterial protein systems co-operate and coordinate multiple functions to equip bacteria to survive and cause disease in the hostile host environment. Our long-range goal is to block this uptake system thereby starving the bacterium of essential nutrients and also promoting clearance by the host immune response. Removal of this important bacterial survival mechanism will thwart the ability for to survive as a pathogen and thus decrease the incidence of disease development.
    Subject(s): Research Article ; Biology ; Immunology ; Microbiology
    ISSN: 1553-7366
    E-ISSN: 1553-7374
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Language: English
    In: Antimicrobial agents and chemotherapy, October 2017, Vol.61(10)
    Description: Oxazolidinones are promising candidates for the treatment of infections. We isolated linezolid-resistant strains from H37Rv (Euro-American) and HN878 (East-Asian) strains; resistance frequencies were similar in the two strains. Mutations were identified in ribosomal protein L3 (RplC) and the 23S rRNA (). All mutant strains were cross resistant to sutezolid; a subset was cross resistant to chloramphenicol. Mutations in led to growth impairment and decreased fitness that may limit spread in clinical settings.
    Subject(s): Mycobacterium ; Mycobacterium Tuberculosis ; Antibiotic Resistance ; Fitness ; Linezolid ; Mycobacteria ; Oxazolidinones ; Resistance ; Ribosome ; Tuberculosis ; Drug Resistance, Multiple, Bacterial -- Genetics ; Linezolid -- Pharmacology ; Mycobacterium Tuberculosis -- Drug Effects ; Protein Synthesis Inhibitors -- Pharmacology ; RNA, Ribosomal, 23s -- Genetics ; Ribosomal Proteins -- Genetics
    ISSN: 0066-4804
    E-ISSN: 1098-6596
    Source: MEDLINE/PubMed (U.S. National Library of Medicine)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Language: English
    Description: Type I IFNs are key cytokines mediating innate antiviral immunity. cGMP-AMP synthase, ritinoic acid-inducible protein 1 (RIG-I)-like receptors, and Toll-like receptors recognize microbial double-stranded (ds)DNA, dsRNA, and LPS to induce the expression of type I IFNs. These signaling pathways converge at the recruitment and activation of the transcription factor IRF-3 (IFN regulatory factor 3). The adaptor proteins STING (stimulator of IFN genes), MAVS (mitochondrial antiviral signaling), and TRIF (TIR domain-containing adaptor inducing IFN-β) mediate the recruitment of IRF-3 through a conserved pLxIS motif. Here we show that the pLxIS motif of phosphorylated STING, MAVS, and TRIF binds to IRF-3 in a similar manner, whereas residues upstream of the motif confer specificity. The structure of the IRF-3 phosphomimetic mutant S386/396E bound to the cAMP response element binding protein (CREB)-binding protein reveals that the pLxIS motif also mediates IRF-3 dimerization and activation....
    Subject(s): Humans ; Rotavirus ; Rotavirus Infections ; Adaptor Proteins ; Signal Transducing ; Membrane Proteins ; Adaptor Proteins ; Vesicular Transport ; Viral Nonstructural Proteins ; Amino Acid Motifs ; Interferon Regulatory Factor-3 ; Creb-Binding Protein ; Immunity ; Innate ; Immune Evasion ; Protein Domains ; Crystal Structure ; Innate Immunity ; Signaling ; Transcription Factor
    Source: eScholarship (California Digital Library)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    In: FEBS Journal, June 2017, Vol.284(11), pp.1631-1643
    Description: Ribosomal protein L7/L12 is associated with translation initiation, elongation, and termination by the 70S ribosome. The guanosine 5′ triphosphate hydrolase (ase) activity of elongation factor G (‐G) requires the presence of L7/L12, which is critical for ribosomal translocation. Here, we have developed new methods for the complete depletion of L7/L12 from 70S ribosomes to analyze the effect of L7/L12 on the activities of the ase factors ‐G, 3, 2, and LepA. Upon removal of L7/L12 from ribosomes, the ase activities of ‐G, 3, and 2 decreased to basal levels, while the activity of LepA decreased marginally. Upon reconstitution of ribosomes with recombinant L12, the ase activities of all ases returned to full activity. Moreover, ribosome binding assays indicated that ‐G, 3, and 2 require L7/L12 for stable binding in the state, and LepA retained 〉 50% binding. Lastly, an ‐G∆G′ truncation mutant possessed ribosome‐dependent ase activity, which was insensitive to L7/L12. Our results indicate that L7/L12 is required for stable binding of ribosome‐dependent ases that harbor direct interactions to the L7/L12 C‐terminal domains, either through a G′ domain (‐G, 3) or a unique N‐terminal domain (2). Furthermore, we hypothesize this interaction is concomitant with counterclockwise ribosomal intersubunit rotation, which is required for translocation, initiation, and post‐termination. The ribosomal protein L7/L12 is thought to be important for translation initiation, elongation and termination by the 70S ribosome. However, its precise role in these reactions was unclear. In this study, Spiegel and colleagues develop methods to unambiguously study the importance of L7/L12 in translation. They show that the presence of L7/L12 is required for the binding and activity of GTPases that form direct contacts with L7/L12, including EF‐G, RF3, and IF2. In contrast, the GTPase LepA, which does not make direct contacts with L7/L12, remains active in its absence. These results suggest that L7/L12 is required for stable binding of ribosome‐dependent GTPases that make direct interactions with L7/L12.
    Subject(s): Ribosome ; Translation ; Elongation Factor G ; Protein L7/L12 ; Gtp Ase
    ISSN: 1742-464X
    E-ISSN: 1742-4658
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Language: English
    In: ProQuest Dissertations and Theses
    Description: Staphylcoccus epidermidis is a ubiquitous commensal of the human skin with few virulence factors; however once inside the body it can form robust biofilms, that are virtually resistant to antibiotic treatment, on indwelling medical devices. Accumulation associated protein (Aap) is a S. epidermidis cell-wall associated protein (CWA) that plays an important role in mediating cell-cell adhesion within the biofilm. Specifically, the B-repeat regions –comprised of extended, ribbon-like ß-sheet modules –overlap from two adjacent cells and form a zinc-zipper with two zinc ions coordinated between each set of anti-parallel B-repeats. This work describes important new findings that refine our understanding of Aap-mediated accumulation of bacterial cells. Though highly identical, Aap B-repeat sequences contain a region of conserved variant amino acids in some of the repeats, but not in others. Interestingly, the alternative set of residues is clustered at the most critical region of the protein –the...
    Subject(s): Biochemistry ; Biochemistry ; Pure Sciences ; Aap ; Biofilm ; Dimerization ; Intercellular Adhesion ; Stability ; Staphylococcus
    ISBN: 978-0-438-39094-2
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
    Source: ProQuest Dissertations & Theses Global: The Sciences and Engineering Collection
    Source: ProQuest Dissertations and Theses Global A&I: The Sciences and Engineering Collection
    Source: ProQuest Dissertations & Theses Full Text
    Source: ProQuest Dissertations & Theses A&I
    Source: ProQuest Dissertations & Theses: Global
    Source: ProQuest One Academic
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Book
    Book
    2008
    ISBN: 9783839408339 
    Language: German
    Description: Der Horrorfilm lässt Erscheinungsformen des Körpers sichtbar werden, die in übrigen Diskursen meist ausgeschlossen oder verworfen werden. Diese genrespezifischen Strategien der Visualisierung zeigen ein »Unzeigbares«, das nicht nur zum abweichenden Gegenbild des Körpers, sondern auch zum Faszinosum und Spektakel erhoben wird. Die Studie deutet diese Körperbilder vor dem Hintergrund kultureller Konzeptionen des Körperlichen und erschließt die heterogenen und historischen Dimensionen, die sich in ihnen einzeichnen. Im Zentrum stehen dabei die Themenfelder Monstrosität, phantastische Krankheit und Tod.
    Subject(s): Horrorfilm ; Körper ; Tod ; Krankheit ; Monstrum ; Film ; Medienwissenschaft ; Body ; Media Studies
    ISBN: 9783839408339
    Source: DOAB (OAPEN Foundation)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    Language: English
    In: ProQuest Dissertations and Theses
    Description: The focus of this study is the prehistory of Western Chiriqu(')i Province, Panama during the Formative Period (ca. 300 B.C. to A.D. 800). Earlier research (Linares and Ranere 1980) demonstrated that a subsistence strategy based on seed (maize) agriculture had replaced earlier hunter-gatherer and/or vegeculture (root crop agriculture) strategies in the late first millennium B.C., but the place of origin for this strategy, its entry point into the region, the exact timing of the replacement and the mechanism(s) responsible for its introduction remained matters of conjecture. Consequently, a program of archaeological survey and test excavations in the mid-elevation and coastal plain zones of Western Chiriqu(')i was designed to examine these questions. From January through August, 1979, this archaeological survey and testing program was conducted along the R(')io Chiriqu(')i Viejo and at San Vincente in Chiriqu(')i. The survey region was divided into three ecological zones along the river....
    Subject(s): Archaeology ; Archaeology ; Social Sciences
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
    Source: ProQuest Dissertations & Theses Global: The Humanities and Social Sciences Collection
    Source: ProQuest Dissertations and Theses Global A&I: The Humanities and Social Sciences Collection
    Source: ProQuest Dissertations & Theses Full Text
    Source: ProQuest Dissertations & Theses A&I
    Source: ProQuest Dissertations & Theses: Global
    Source: ProQuest One Academic
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...