Molecular Breeding, 2013, Vol.31(4), pp.909-920
To facilitate marker-assisted breeding and genetic analyses of pepper ( Capsicum annuum ), we developed non-redundant 2- or 3-base simple sequence repeat (SSR) markers from enriched C. annuum genomic libraries and from C. annuum cDNA sequences in public databases. The SSR-enriched libraries were constructed using combinations of three restriction enzymes ( Alu I, Hae III, and Rsa I) and two biotinylated oligonucleotides [b(GA) 15 and b(CA) 15 ]. Ultimately, we obtained 1,736 genomic SSR markers and 1,344 cDNA-derived SSR markers from 6,528 clones and 13,003 sequences, respectively. We mapped 597 markers, including 265 of the newly developed SSR markers, onto a linkage map by using doubled-haploid (DH) lines derived from an intraspecific cross of two pure lines of C . annuum (K9-11 × MZC-180). The map, designated as the KL-DH map, consisted of 12 linkage groups. The map covered a genetic distance of 2,028 cM, and the average distance between markers was less than 4 cM. The frame structure of the KL-DH map was compared with the published standard conserved ortholog set II (COSII) map, which was derived from an interspecific F 2 population ( C. frutescens × C. annuum ), by using tomato ( Solanum lycopersicum ) chromosomal sequences to bridge the two maps. The intraspecific KL-DH map constructed in this study and the interspecific COSII map were similar in map length and marker distribution, suggesting that the KL-DH map covers nearly the whole genome of C. annuum .
Capsicum ; Linkage map ; SSR ; Tomato genome
Permalink to record
View full text in Springer (Subscribers only)