The Journal of biological chemistry, 29 December 2017, Vol.292(52), pp.21490-21503
The Ig-like cell adhesion molecule (IgCAM) BT-IgSF (brain- and testis-specific Ig superfamily protein) plays a major role in male fertility in mice. However, the molecular mechanism by which BT-IgSF supports fertility is unclear. Here, we found that it is localized in Sertoli cells at the blood-testis barrier (BTB) and at the apical ectoplasmic specialization. The absence of BT-IgSF in Sertoli cells in both global and conditional mouse mutants ( AMHCre and Rosa26CreERT2 lines) resulted in male infertility, atrophic testes with vacuolation, azoospermia, and spermatogenesis arrest. Although transcripts of junctional proteins such as connexin43, ZO-1, occludin, and claudin11 were up-regulated in the absence of BT-IgSF, the functional integrity of the BTB was impaired, as revealed by injection of a BTB-impermeable component into the testes under conditions. Disruption of the BTB coincided with mislocalization of connexin43, which was present throughout the seminiferous epithelium and not restricted...
Bt-Igsf (Igsf11) ; Igcam ; Igcam Bt-Igsf (Igsf11) ; Blood–Testis Barrier ; Cell Adhesion ; Connexin43 ; Male Fertility ; Spermatogenesis ; Testis ; Blood-Testis Barrier -- Physiology ; Immunoglobulins -- Physiology ; Spermatogenesis -- Genetics
Permalink to record
View this record in MEDLINE/PubMed