placeholder
and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Proceed order?

Export
Filter
Language
Year
  • 1
    Language: English
    In: Proceedings of the National Academy of Sciences of the United States of America, 19 December 2017, Vol.114(51), pp.13471-13476
    Description: Cells experience compressive stress while growing in limited space or migrating through narrow constrictions. To survive such stress, cells reprogram their intracellular organization to acquire appropriate mechanical properties. However, the mechanosensors and downstream signaling networks mediating these changes remain largely unknown. Here, we have established a microfluidic platform to specifically trigger compressive stress, and to quantitatively monitor single-cell responses of budding yeast in situ. We found that yeast senses compressive stress via the cell surface protein Mid2 and the calcium channel proteins Mid1 and Cch1, which then activate the Pkc1/Mpk1 MAP kinase pathway and calcium signaling, respectively. Genetic analysis revealed that these pathways work in parallel to mediate cell survival. Mid2 contains a short intracellular tail and a serine-threonine-rich extracellular domain with spring-like properties, and both domains are required for mechanosignaling. Mid2-dependent...
    Subject(s): Pkc1/Mpk1 Pathway ; Calcium Signaling ; Cell Polarity ; Mechanosensor ; Microfluidics ; Stress, Mechanical ; Calcineurin -- Metabolism ; Calcium Channels -- Metabolism ; Protein Kinase C -- Metabolism ; Saccharomyces Cerevisiae Proteins -- Metabolism
    ISSN: 0027-8424
    E-ISSN: 1091-6490
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    In: Journal of Food Science, August 2013, Vol.78(8), pp.C1144-C1151
    Description: Lipid oxidation and antioxidant degradation in dried laver () were determined during storage at water activities () of 0.11, 0.30, 0.51, 0.75, or 0.89 in the dark at 40 °C for 15 d. Lipid oxidation was evaluated by measuring peroxide value (POV) and conjugated dienoic acid (CDA) contents, and fatty acid composition was analyzed by gas chromatography. Contents of polyphenols, tocopherols, and porphyran were determined by spectrophotometry, HPLC, and gravimetry, respectively. The POV and CDA contents of the dried laver lipids increased during storage as increased from 0.11 to 0.30, 0.51, 0.75, and 0.89, whereas the relative content of eicosapentaenoic acid was decreased; however, the contents of polyphenols, α‐tocopherol, and porphyran in dried laver showed the reverse phenomena. Lipid oxidation and antioxidant degradation in dried laver sharply increased at an of 0.51. Polyphenols, α‐tocopherol, and porphyran contributed to reduction of lipid oxidation in dried laver. The degree of lipid oxidation of dried laver was more dependent on the concentration of α‐tocopherol than that of either polyphenols or porphyran during storage in the dark. The results strongly suggest that the quality of dried laver can be improved by preserving tocopherols as much as possible while decreasing during storage. The results of this study can be applied to improve shelf life of dried seaweeds with high contents of antioxidants.
    Subject(s): Antioxidant Degradation ; Lipid Oxidation ; Dried Laver ; Water Activity
    ISSN: 0022-1147
    E-ISSN: 1750-3841
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Language: English
    In: International Journal of Production Research, 03 August 2014, Vol.52(15), pp.4488-4506
    Description: Firms need to find a proper fit between their own information technology (IT) capabilities and IT systems in order to enhance performance. Thus, the purpose of this paper is to examine the moderating effect of certain e-procurement system types on the relationship between IT capabilities and firm performance. Moderated regression analysis is used to test for interaction effects of IT capabilities, including collaboration capability and dynamic capability, and e-procurement system types including, E-market and Extranet. Data of 142 Korean manufacturing firms are collected and used in the analysis. There is a stronger effect when relationships between IT capability and firm performance are considered with the involvement of other resource such as e-procurement system type. When e-procurement has a minimum-information sharing function and a high market-making function, collaboration capability had a greater contribution on financial performance. On the other hand, when an e-procurement...
    Subject(s): Managerial It Capability ; E-Procurement System ; Firm Performance ; Resource-Based View ; Moderating Effect ; Engineering
    ISSN: 0020-7543
    E-ISSN: 1366-588X
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Language: English
    In: 2012, Vol.7(8), p.e42983
    Description: Even though the disruption of axonal transport is an important pathophysiological factor in neurodegenerative diseases including Alzheimer's disease (AD), the relationship between disruption of axonal transport and pathogenesis of AD is poorly understood. Considering that α-tubulin acetylation is an important factor in axonal transport and that Aβ impairs mitochondrial axonal transport, we manipulated the level of α-tubulin acetylation in hippocampal neurons with Aβ cultured in a microfluidic system and examined its effect on mitochondrial axonal transport. We found that inhibiting histone deacetylase 6 (HDAC6), which deacetylates α-tubulin, significantly restored the velocity and motility of the mitochondria in both anterograde and retrograde axonal transports, which would be otherwise compromised by Aβ. The inhibition of HDAC6 also recovered the length of the mitochondria that had been shortened by Aβ to a normal level. These results suggest that the inhibition of HDAC6 significantly rescues hippocampal neurons from Aβ-induced impairment of mitochondrial axonal transport as well as mitochondrial length. The results presented in this paper identify HDAC6 as an important regulator of mitochondrial transport as well as elongation and, thus, a potential target whose pharmacological inhibition contributes to improving mitochondrial dynamics in Aβ treated neurons.
    Subject(s): Research Article ; Biology ; Medicine ; Biotechnology ; Cell Biology ; Neuroscience ; Neurological Disorders ; Biochemistry
    E-ISSN: 1932-6203
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    In: International journal of production research, 2014, Vol.52(15), p.4488
    ISSN: 0020-7543
    Source: wiso Wirtschaftswissenschaften (GBI-Genios Deutsche Wirtschaftsdatenbank GmbH) 〈img src="http://exlibris-pub.s3.amazonaws.com/wiso_logo.jpg" style="vertical-align:middle;margin-left:7px"〉
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    In: International Journal of Production Research, 2014, Vol.52(15), p.4488-4506
    Subject(s): Informationstechnologie
    ISSN: 0020-7543
    Source: wiso Wirtschaftswissenschaften (GBI-Genios Deutsche Wirtschaftsdatenbank GmbH) 〈img src="http://exlibris-pub.s3.amazonaws.com/wiso_logo.jpg" style="vertical-align:middle;margin-left:7px"〉
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    In: Journal of the Science of Food and Agriculture, September 2015, Vol.95(12), pp.2409-2415
    Description: To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1002/jsfa.6961/abstract Byline: Mak-Soon Lee, Sunyoon Jung, Soojung Oh, Yoonjin Shin, Chong-Tai Kim, In-Hwan Kim, Yangha Kim Keywords: fresh ginseng; high hydrostatic pressure extract; adipogenesis; 3T3-L1 cells; obesity Abstract BACKGROUND Red ginseng is produced by steaming and drying fresh ginseng. Through this processing, chemical compounds are modified, and then biological activities are changed. In the food-processing industry, high hydrostatic pressure (HHP) has become an alternative to heat processing to make maximum use of bioactive compounds in food materials. This study comparatively investigated the anti-adipogenic effects of water extract of red ginseng (WRG) and high hydrostatic pressure extract of fresh ginseng (HPG) in 3T3-L1 adipocytes. RESULTS Both WRG and HPG inhibited the accumulation of intracellular lipids and triglycerides, and the activity of glycerol-3-phosphate dehydrogenase (GPDH), a key enzyme in triglyceride biosynthesis. Intracellular lipid content and GPDH activity were significantly lower in the HPG group compared to the WRG group. In addition, mRNA expression of adipogenic genes, including CEBP-[alpha], SREBP-1c and aP2, were lower in HPG-treated cells compared to WRG-treated cells. HPG significantly increased the activity of AMPK, and WRG did not. CONCLUSION Results suggested that HPG may have superior beneficial effects on the inhibition of adipogenesis compared with WRG. The anti-adipogenic effects of HPG were partially associated with the inhibition of GPDH activity, suppression of adipogenic gene expression and activation of AMPK in 3T3-L1 adipocytes. [c] 2014 Society of Chemical Industry
    Subject(s): Fresh Ginseng ; High Hydrostatic Pressure Extract ; Adipogenesis ; 3t3‐L1 Cells ; Obesity
    ISSN: 0022-5142
    E-ISSN: 1097-0010
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Language: English
    In: Food Science and Biotechnology, 12/2013, Vol.22(6), pp.1523-1529
    Description: Byline: Soojung Oh (1), Malshick Shin (2), Kyungae Lee (3), Eunok Choe (1) Keywords: dried laver; water activity; chlorophyll; carotenoid; phycobilin Abstract: Degradation of chlorophyll, carotenoids, and phycobilins in dried laver (Porphyra) was studied during storage at water activities (Aw) of 0.112, 0.316, 0.484, 0.747, or 0.890 in the dark at 40degC for 15 days. The chlorophyll, carotenoid, and phycobilin contents were determined using HPLC and spectrophotometry. The chorophyll a, carotenoid, and phycobilin contents in dried laver decreased with storage time in the dark, and degradation was increased and accelerated as the Aw value increased. Among pigments, chlorophyll a was degraded at the highest rate, and differences in degradation rates among pigments became greater as the Aw value increased. Phycoerythrin was more stable than phycocyanin. Changes in the Aw value affected degradation of phycocyanin and chlorophyll more than phycoerythrin or carotenoids. Control of the Aw value can improve the color stability of dried laver in the dark. Author Affiliation: (1) Department of Food and Nutrition, Inha University, Incheon, 402-751, Korea (2) Department of Food and Nutrition, Chonnam National University, Gwangju, 500-757, Korea (3) Department of Food and Nutrition, Soonchunhyang University, Asan, 336-745, Korea Article History: Registration Date: 17/10/2013 Received Date: 08/02/2013 Accepted Date: 24/05/2013 Online Date: 17/10/2013
    Subject(s): Chlorophyll -- Analysis ; Chromophores -- Analysis ; Carotenoids -- Analysis;
    ISSN: 1226-7708
    E-ISSN: 2092-6456
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Language: English
    In: Food Science and Biotechnology, 2014, Vol.23(3), pp.701-709
    Description: Effects of light on lipid oxidation, antioxidants, and pigments in dried laver ( Porphyra ) were studied during storage at 40°C and at a water activity of 0.75 for 15 days. Lipid oxidation was evaluated by measuring peroxide value (POV) and conjugated dienoic acid (CDA) contents, whereas fatty acid composition was analyzed by gas chromatography. Contents of polyphenols, tocopherols, porphyran, chlorophylls, carotenoids, and phycobilins were also monitored. The POV and CDA contents of dried laver lipids increased during storage, whereas contents of eicosapentaenoic acid, pigments, and antioxidants decreased. Light accelerated lipid oxidation as well as degradation of antioxidants and pigments during storage of dried laver. Chlorophyll and polyphenols were the most rapidly degraded among all pigments and antioxidants, respectively, and light had the strongest effect on their degradation. Lipid oxidation of dried laver due to light was highly dependent on the content of a-tocopherol among all minor compounds.
    Subject(s): dried laver ; light ; pigments ; antioxidants ; lipid oxidation
    ISSN: 1226-7708
    E-ISSN: 2092-6456
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    In: Lab on a Chip, 2015, Vol.15(20), pp.3984-3988
    Description: Current in vitro systems mimicking bone tissues fail to fully integrate the three-dimensional (3D) microvasculature and bone tissue microenvironments, decreasing their similarity to in vivo conditions. Here, we propose 3D microvascular networks in a hydroxyapatite (HA)-incorporated extracellular matrix (ECM) for designing and manipulating a vascularized bone tissue model in a microfluidic device. Incorporation of HA of various concentrations resulted in ECM with varying mechanical properties. Sprouting angiogenesis was affected by mechanically modulated HAextracellular matrix interactions, generating a model of vascularized bone microenvironment. Using this platform, we observed that hydroxyapatite enhanced angiogenic properties such as sprout length, sprouting speed, sprout number, and lumen diameter. This new platform integrates fibrin ECM with the synthetic bone mineral HA to provide in vivo -like microenvironments for bone vessel sprouting.
    Subject(s): Mimicry ; Microvasculature ; Hydroxyapatite ; Microfluidics ; Extracellular Matrix ; Fibrin ; Angiogenesis ; Minerals ; Mechanical Properties ; Models ; Bone and Bone Diseases;
    ISSN: 1473-0197
    E-ISSN: 1473-0189
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...