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  • 1
    Language: English
    In: Fertility and sterility, 2016, Vol.105 (6), p.1589-1593
    Description: Objective To determine the best practices of intrauterine insemination with the partner's fresh sperm. Design Prospective multicenter observational study. Setting Assisted reproduction technology (ART) centers. Patient(s) Seven hundred and seven patients entering the program, regardless of age or cause of infertility. Intervention(s) Intrauterine insemination by standard procedures. Main Outcome Measure(s) Effect of patient characteristics (duration of infertility, indications, age, parity, body mass index, semen parameters) as well as IUI parameters on delivery rates per couple or per attempt. Result(s) The overall live birth rate was 11.4% per cycle, varying from 8.4% to 17.6% between centers. The main differences in practice that had a statistically significant impact on the delivery rate were the use of gonadotropin-releasing hormone (GnRH) antagonists (15.2% with versus 9.4% without) and the number of mature recruited follicles (9.4% for one versus 15.2% for two). Conclusion(s) Our results indicate that the use of GnRH antagonists has a positive effect on the delivery rate, especially in the multifollicular stimulations that are required when women are older than 27 years.
    Subject(s): Internal Medicine ; Obstetrics and Gynecology ; Delivery ; IUI ; GnRH antagonist ; ovarian stimulation ; France - epidemiology ; Prospective Studies ; Humans ; Middle Aged ; Reproductive Techniques, Assisted - standards ; Male ; Gonadotropin-Releasing Hormone - antagonists & inhibitors ; Insemination, Artificial - trends ; Hormone Antagonists - pharmacology ; Pregnancy ; Young Adult ; Insemination, Artificial - standards ; Insemination, Artificial - methods ; Adult ; Female ; Hormone Antagonists - therapeutic use ; Pregnancy Rate - trends ; Reproductive Techniques, Assisted - trends ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 2
    Language: English
    In: Reproductive biology and endocrinology, 2016-05-21, Vol.14 (1), p.28-28
    Description: Follicular fluid (FF) is an important micro-environment influencing oocyte growth, its development competence, and embryo viability. The FF content analysis allows to identify new relevant biomarkers, which could be predictive of in vitro fertilization (IVF) outcomes. Inside ovarian follicle, the amount of FF components from granulosa cells (GC) secretion, could be regulated by gonadotropins, which play a major role in follicle development. This prospective study included 61 female undergoing IVF or Intra-cytoplasmic sperm injection (ICSI) procedure. Apolipoprotein B (APOB) concentrations in follicular fluid and APOB gene and protein expression in granulosa cells from reproductively aged women undergoing an in vitro fertilization program were measured. The statistical analyses were performed according to a quartile model based on the amount of APOB level found in FF. Amounts of APOB were detected in human FF samples (mean ± SD: 244.6 ± 185.9 ng/ml). The odds of obtaining an oocyte in the follicle and a fertilized oocyte increased significantly when APOB level in FF was higher than 112 ng/ml [i.e., including in Quartile Q 2, Q3 and Q4] (p = 0.001; p 〈 0.001, respectively). The probabilities of obtaining an embryo and a top quality embryo on day 2, were significantly higher if APOB levels were within the ranges of 112 and 330 ng/ml (i.e. in Q2 and Q3) or 112 and 230 ng/ml (i.e. in Q2), respectively (p 〈 0.001; p = 0.047, respectively). In addition, our experiments in vitro indicated that APOB gene and protein expression, along with APOB content into culture were significantly under-expressed in GC upon stimulation with gonadotropins (follicular stimulating hormone: FSH and/or human chorionic gonadotropin: hCG). We are reporting a positive and statistically significant associations between APOB and oocyte retrieval, oocyte fertilization, and embryo quality. Using an experimental study component, the authors report significant reduced APOB expression and content for luteinized granulosa cells cultured in the presence of gonadotropins.
    Subject(s): Biomarkers - metabolism ; Apolipoproteins B - metabolism ; Prospective Studies ; Fertilization ; Ovulation Induction ; Embryo, Mammalian ; Humans ; Treatment Outcome ; Follicular Fluid - metabolism ; Embryonic Development ; Oocyte Retrieval ; Granulosa Cells - metabolism ; Chorionic Gonadotropin - therapeutic use ; Fertilization in Vitro ; Follicle Stimulating Hormone - therapeutic use ; Adult ; Female ; Fertilization in vitro ; Chorionic gonadotropin ; Analysis ; Influence ; Research ; Apolipoproteins ; Health aspects ; Index Medicus ; Life Sciences
    ISSN: 1477-7827
    E-ISSN: 1477-7827
    Source: BioMedCentral Open Access
    Source: Academic Search Ultimate
    Source: PubMed Central
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  • 3
    Language: English
    In: Fertility and sterility, 2008, Vol.90 (5), p.1792-1799
    Description: Objective To assess the value of sperm DNA fragmentation, measured by the sperm chromatin dispersion (SCD) test, in predicting fertilization rate, embryo quality, and pregnancy outcome. Design Prospective study. Setting Four French infertility centers, from January to August 2005. Patient(s) Six hundred twenty-two couples participating in their first IVF or ICSI program. Intervention(s) Analysis of DNA fragmentation by the sperm chromatin dispersion test in sperm samples used for IVF or ICSI. Main Outcome Measure(s) Correlations and associations between sperm parameters, sperm DNA integrity, and pregnancy outcomes. Result(s) A statistically significant correlation was observed between sperm DNA fragmentation rate and the following sperm characteristics: sperm motility, morphology, and concentration. We found a statistically significant relationship between sperm DNA fragmentation rate and fertilization rate, and we were able to suggest a threshold sperm DNA fragmentation rate of 18%, above which fragmentation rate was predictive of fertilization rate. Regarding embryo quality, we observed a relationship between sperm DNA fragmentation and embryo quality. No significant relationship was found between sperm DNA fragmentation rate and clinical pregnancies or births. Conclusion(s) The results of this study confirm the utility of the sperm chromatin dispersion test for assessment of DNA fragmentation.
    Subject(s): Internal Medicine ; Obstetrics and Gynecology ; IVF ; ICSI ; SCD test ; infertility ; DNA fragmentation ; Gynecology. Andrology. Obstetrics ; Birth control ; Biological and medical sciences ; Medical sciences ; Sterility. Assisted procreation ; Sperm Motility ; Sperm Count ; Reproducibility of Results ; Spermatozoa - pathology ; Prospective Studies ; Infertility - pathology ; Humans ; Chromatin Assembly and Disassembly ; Male ; Pregnancy ; Sperm Injections, Intracytoplasmic ; Fertilization in Vitro ; Cell Shape ; DNA Fragmentation ; Adult ; Female ; France ; Infertility - therapy ; Pregnancy Outcome ; Semen Analysis - methods ; Spermatozoa ; Chromatin ; DNA ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 4
    Language: English
    In: Molecular human reproduction, 2007-06, Vol.13 (6), p.373-379
    Description: Progesterone production by luteal cells is dependent on the supply of cholesterol by lipoproteins. The aim of this study was to determine whether the liver X receptors (LXRs) contribute to cholesterol homeostasis and progesterone secretion in human luteinized granulosa cells. Cells were isolated from follicular aspirates of patients undergoing in vitro fertilization. Luteinization was induced by a 7-day treatment with human chorionic gonadotrophin. LXR beta was expressed at higher levels than LXR alpha in granulosa cells and its expression was increased during luteinization. Treatment of luteinized granulosa cells by LXR agonists induced a significant time- and dose-dependent reduction in progesterone secretion (50% reductions after a 7-day treatment with 1-µM of either GW3965 or T0901317). mRNA levels of steroidogenic genes including steroidogenic acute regulatory protein and P450 side-chain cleavage were only moderately affected by LXR activation, with a significant reduction that was observed at 10 µM agonist concentration. Cellular cholesterol was markedly reduced after treatment with LXR agonists as a result of an increased cholesterol efflux that was related to the induction of LXR target genes (ABCA1, ABCG1, apo E, PLTP). Our study identifies LXRs as new, key actors contributing to regulation of cholesterol metabolism and steroidogenesis in luteinized granulosa cells.
    Subject(s): Liver X receptor ; cholesterol ; progesterone ; Fundamental and applied biological sciences. Psychology ; Embryology: invertebrates and vertebrates. Teratology ; Biological and medical sciences ; Humans ; Gene Expression Regulation ; Receptors, Cytoplasmic and Nuclear - agonists ; Luteinization - drug effects ; Cholesterol - metabolism ; Hydrocarbons, Fluorinated ; Phosphoproteins - metabolism ; RNA, Messenger - metabolism ; Sulfonamides - pharmacology ; Granulosa Cells - drug effects ; Granulosa Cells - metabolism ; DNA-Binding Proteins - metabolism ; Protein Isoforms - metabolism ; DNA-Binding Proteins - agonists ; Liver X Receptors ; Benzoates - pharmacology ; Female ; Progesterone - biosynthesis ; Orphan Nuclear Receptors ; Benzylamines - pharmacology ; Receptors, Cytoplasmic and Nuclear - metabolism ; Index Medicus
    ISSN: 1360-9947
    E-ISSN: 1460-2407
    Source: Alma/SFX Local Collection
    Source: Oxford Journals 2016 Current and Archive A-Z Collection
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  • 5
    Language: English
    In: PloS one, 2016, Vol.11 (3), p.e0150857-e0150857
    Description: In animal studies, extensive data revealed the influence of culture medium on embryonic development, foetal growth and the behaviour of offspring. However, this impact has never been investigated in humans. For the first time, we investigated in depth the effects of embryo culture media on health, growth and development of infants conceived by In Vitro Fertilization until the age of 5 years old. This single-centre cohort study was based on an earlier randomized study. During six months, in vitro fertilization attempts (No. 371) were randomized according to two media (Single Step Medium--SSM group) or Global medium (Global group). This randomized study was stopped prematurely as significantly lower pregnancy and implantation rates were observed in the SSM group. Singletons (No. 73) conceived in the randomized study were included (42 for Global and 31 for SSM). The medical data for gestational, neonatal and early childhood periods were extracted from medical records and parental interviews (256 variables recorded). The developmental profiles of the children in eight domains (social, self-help, gross motor, fine motor, expressive language, language comprehension, letter knowledge and number knowledge--270 items) were compared in relation to the culture medium. The delivery rate was significantly lower in the SSM group than in the Global group (p〈0.05). The culture medium had no significant effect on birthweight, risk of malformation (minor and major), growth and the frequency of medical concerns. However, the children of the Global group were less likely than those of the SSM group to show developmental problems (p = 0.002), irrespective of the different domains. In conclusion, our findings showed that the embryo culture medium may have an impact on further development.
    Subject(s): Fertilization in Vitro ; Culture Media ; Growth ; Humans ; Child, Preschool ; Infant ; Health Status ; Infant, Newborn ; Cohort Studies ; Fertilization in vitro ; Culture media (Biology) ; Embryonic development ; Research ; Child development ; Analysis ; Index Medicus ; Life Sciences ; Human health and pathology ; Gynecology and obstetrics ; Cognitive science ; In vitro fertilization ; Neonates ; Fertilization ; Sperm ; Medical records ; Implantation ; Cardiovascular disease ; Infants ; Gene expression ; Pregnancy ; Embryonic growth stage ; Autism ; Embryogenesis ; Offspring ; Randomization ; Language ; DNA methylation ; Culture media ; Epigenetics ; Birth weight ; Reproductive technologies ; Blood pressure ; Children
    ISSN: 1932-6203
    E-ISSN: 1932-6203
    Source: Academic Search Ultimate
    Source: PubMed Central
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  • 6
    Language: English
    In: The FASEB journal, 2006-04, Vol.20 (6), p.794-796
    Description: ABSTRACT Vitamin E was discovered for its implication in reproductive biology, and its transport in mammalian plasma and brain was shown to be governed by plasma phospholipid transfer protein (PLTP). We show that PLTP deficiency is associated with hypofertility of mouse males but not mouse females, and it accounts for a significant decrease in total number of pups produced over a 2‐month breeding period of PLTP knocked out mice (−32%, P〈0.03). PLTP is highly expressed in epididymis of mouse males, and α‐tocopherol, the main vitamin E isomer in vivo, was significantly less abundant in cauda and caput epididymis of PLTP‐deficient mice as compared with wild‐type counterparts (caput: −26%, P〈0.05; cauda: −21%, P〈0.05). Mature spermatozoa from PLTP‐deficient epididymis were shown to retain an abnormal α‐tocopherol content. PLTP deficiency tended to reduce sperm motility as shown by a 24% reduction in spermatozoa with progressive motility (P〈0.02), with no change in other sperm parameters as compared with wild‐type males. Finally, in vitro fertilization rates of wild‐type oocytes with spermatozoa from PLTP‐deficient males were markedly reduced as compared with those measured with spermatozoa from wild‐type males (−60%, P〈0.05). It is concluded that PLTP is a new, key factor that determines sperm motility and male fertility.
    Subject(s): reproductive biology ; alpha‐tocopherol ; vitamin E ; Epididymis - metabolism ; Sperm Motility - genetics ; Male ; Spermatozoa - metabolism ; Phospholipid Transfer Proteins - metabolism ; Phospholipid Transfer Proteins - deficiency ; Animals ; Fertilization in Vitro ; Infertility, Male - genetics ; Phospholipid Transfer Proteins - genetics ; Gene Deletion ; Female ; Infertility, Male - metabolism ; Mice ; alpha-Tocopherol - metabolism ; Index Medicus
    ISSN: 0892-6638
    E-ISSN: 1530-6860
    Source: Federation of American Societies for Experimental Biology
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  • 7
    Language: English
    In: Journal of gynecology obstetrics and human reproduction, 2020-11, Vol.49 (9), p.101902-101902
    Description: France is known for its conservative and unique position in assisted reproductive technologies (ARTs). At the eve of the future revision of French Bioethics laws, we decided to conduct a national survey to examine the opinions of French specialists in ARTs about social issues. Descriptive study conducted in May 2017 in a university teaching hospital using an anonymous online questionnaire on current issues in ARTs. The questionnaire was sent by email to 650 French ARTs specialists, both clinicians and embryologists. After 3 reminders, 408 responses were collected resulting in a participation rate of 62.7% (408/650). Concerning pre-implantation genetic testing, 80% of the physicians were in favor of expanding the indications, which in France are presently limited to incurable genetic diseases. Authorizing elective Fertility Preservation was supported by 93.4% of the specialists, but without social coverage for 86.3% of them. Concerning gamete donation, 77.4% of the French ARTs specialists were in favor of giving a financial compensation to donors, 92% promoted preserving their anonymity and 80.9% were against a directed donation. ARTs for single heterosexual women were supported by 63.4% of the French specialists and by 72.5% for lesbian couples. The legalization of surrogacy was requested by 55.2%. Pending the revision of the French Bioethics laws, this survey provides an overview of the opinion of the specialists in ARTs on expanding ARTs for various social indications.Because of the evolution of social values, a more liberal and inclusive ART program is desired by the majority of ART specialists in France.
    Subject(s): Bioethics ; Gamete donation ; Single women ; France ; Elective oocyte cryopreservation ; Lesbian couples ; Index Medicus
    ISSN: 2468-7847
    E-ISSN: 2468-7847
    Source: Freedom Collection Journals [SCFCJ]
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  • 8
    Language: English
    In: Journal of medical genetics, 2013-04, Vol.50 (4), p.220-227
    Description: Background The high frequency of the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) gene mutation p.Arg117His in patients with congenital bilateral absence of the vas deferens (CBAVD) and in newborns screened for CF has created a dilemma. Methods Phenotypic and genotypic data were retrospectively collected in 179 non-newborn French individuals carrying p.Arg117His and a second CFTR mutation referred for symptoms or family history, by all French molecular genetics laboratories, referring physicians, CF care centres and infertility clinics. Results 97% of the patients had the intronic T7 normal variant in cis with p.Arg117His. 89% patients were male, with CBAVD being the reason for referral in 76%. In 166/179 patients with available detailed clinical features, final diagnoses were: four late-onset marked pulmonary disease, 83 isolated CBAVD, 67 other CFTR-related phenotypes, including 44 CBAVD with pulmonary and/or pancreatic symptoms and 12 asymptomatic cases. Respiratory symptoms were observed in 30% of the patients, but the overall phenotype was mild. No correlation was observed between sweat chloride concentrations and disease severity. Five couples at risk of CF offspring were identified and four benefited from prenatal or preimplantation genetic diagnoses (PND or PGD). Eight children were born, including four who were compound heterozygous for p.Arg117His and one with a severe CF mutation. Conclusions Patients with CBAVD carrying p.Arg117His and a severe CF mutation should benefit from a clinical evaluation and follow-up. Depending on the CBAVD patients’ genotype, a CFTR analysis should be considered in their partners in order to identify CF carrier couples and offer PND or PGD.
    Subject(s): Reproductive medicine ; Cystic fibrosis ; Vas Deferens - pathology ; Humans ; Infertility, Male - complications ; Prenatal Diagnosis ; Vas Deferens - abnormalities ; Cystic Fibrosis - pathology ; Child, Preschool ; Infant ; Male ; Male Urogenital Diseases - complications ; Mutation Rate ; Cystic Fibrosis - complications ; Sweat - chemistry ; Phenotype ; Infertility, Male - genetics ; Cystic Fibrosis - genetics ; Male Urogenital Diseases - pathology ; Cystic Fibrosis Transmembrane Conductance Regulator - genetics ; Female ; Heterozygote ; Mutation ; Child ; Male Urogenital Diseases - genetics ; Infant, Newborn ; Genetic aspects ; Research ; Gene mutations ; Genitourinary organs ; Abnormalities ; Index Medicus ; Life Sciences
    ISSN: 0022-2593
    E-ISSN: 1468-6244
    Source: Alma/SFX Local Collection
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  • 9
    Language: English
    In: Human reproduction (Oxford), 2005-03, Vol.20 (3), p.736-740
    Description: BACKGROUND: Recent data emphasized the implication of polymerase γ (POLG) CAG repeats in infertility, making it a very attractive gene for study. A comparison of POLG CAG repeats in infertile and fertile men showed a clear association between the absence of the usual 10-CAG allele and male infertility, excluding azoospermia. It has also been suggested that the POLG gene polymorphism should be considered as a possible contributing factor in unexplained couple infertility where semen parameters are normal. In this study, we investigated the POLG CAG repeats, in a well-defined population of patients with severe male factor infertility. METHODS: We conducted a large study of POLG CAG repeats in 433 infertile and 91 fertile, normozoospermic and healthy males. In all subjects, phenotypic data, including semen parameters, hormonal status and clinical profiles, were available. RESULTS: Thirteen ‘homozygous mutants’ (3%) were found among the 433 idiopathic infertile patients. The follow-up of the 13 ‘homozygous mutant’ resulted in pregnancy for more than half of the couples, through assisted reproductive techniques or even spontaneously. In addition, one ‘homozygous mutant’ was identified in 91 fertile men (1.1%) CONCLUSION: Under our conditions, our study does not confirm any relationship between the polymorphic CAG repeat in the POLG gene and male infertility.
    Subject(s): male infertility ; CAG repeats ; POLG ; Gynecology. Andrology. Obstetrics ; Biological and medical sciences ; Medical sciences ; DNA Polymerase gamma ; Severity of Illness Index ; Trinucleotide Repeats ; Gene Frequency ; Humans ; Male ; Case-Control Studies ; DNA-Directed DNA Polymerase - genetics ; Reproductive Techniques, Assisted ; Infertility, Male - physiopathology ; Pregnancy ; Homozygote ; DNA, Mitochondrial - genetics ; Infertility, Male - genetics ; Adult ; Female ; Infertility, Male - therapy ; Mutation ; Pregnancy Rate ; Index Medicus ; Life Sciences ; Computer Science
    ISSN: 0268-1161
    E-ISSN: 1460-2350
    Source: Alma/SFX Local Collection
    Source: Oxford Journals 2016 Current and Archive A-Z Collection
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  • 10
    Language: English
    In: Fertility and sterility, 2003, Vol.79, p.1649-1651
    Description: To present the first case of an infertile male with azoospermia related to a congenital bilateral absence of the vas deferens (CBAVD), in which mutations within the cystic fibrosis transmembrane conductance regulator (CFTR) gene coexist with a robertsonian translocation. Case report. A university hospital. A 34-year-old male with a 2-year history of primary infertility. Lymphocytic karyotype, polymerase chain reaction (PCR) and allele oligonucleotide-specific hybridization (ASO), microsurgical epididymal sperm aspiration (MESA), and intracytoplasmic sperm injection (ICSI). Physical examination and semen analysis. Semen analyses revealed azoospermia and a well-recognized obstructive phenotype. Analysis of the CFTR gene revealed a compound heterozygosity for a 2184 del A + 2183 A → G mutation on one allele and the 5T variant within the polypyrimidine tract of intron 8 on the other allele. Cytogenetic analyses revealed a t(13;14)(q10;q10) robertsonian translocation in the same patient. Microsurgical epididymal sperm aspiration allowed retrieval of a million mature motile spermatozoa, excluding any spermatogenic impairment secondary to the genetic abnormalities found in this patient. Epididymal sperm was used for an intracytoplasmic sperm injection program, and a normal child was born at term. This case illustrates that two distinct genetic defects may coexist, with a variable effect on male fertility but with important implications for genetic counseling of the future pregnancy.
    Subject(s): robertsonian translocation ; Azoospermia ; infertility ; CFTR ; CBAVD ; Gynecology. Andrology. Obstetrics ; Biological and medical sciences ; Medical sciences ; Non tumoral diseases ; Male genital diseases ; Sperm Injections, Intracytoplasmic ; Translocation, Genetic ; Humans ; Vas Deferens - abnormalities ; Chromosomes, Human, Pair 13 ; Adult ; Chromosomes, Human, Pair 14 ; Cystic Fibrosis Transmembrane Conductance Regulator - genetics ; Male ; Infant, Newborn ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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