The Plant cell, 2009-12-01, Vol.21 (12), p.3984-4001
Plastid-targeted proteins pass through the cytosol as unfolded precursors. If proteins accumulate in the cytosol, they can form nonspecific aggregates that cause severe cellular damage. Here, we demonstrate that high levels of plastid precursors are degraded through the ubiquitin-proteasome system (UPS) in Arabidopsis thaliana cells. The cytosolic heat shock protein cognate 70-4 (Hsc70-4) and E3 ligase carboxy terminus of HscTO-interacting protein (CHIP) were highly induced in plastid protein import2 plants, which had a T-DNA insertion at Toc159 and showed an albino phenotype and a severe defect in protein import into chloroplasts. Hsc70-4 and CHIP together mediated plastid precursor degradation when import-defective chloroplast-targeted reporter proteins were transiently expressed in protoplasts. Hsc70-4 recognized specific sequence motifs in transit peptides and thereby led to precursor degradation through the UPS. CHIP, which interacted with Hsc70-4, functioned as an E3 ligase in the Hsc70-4-mediated protein degradation. The physiological role of Hsc70-4 was confirmed by analyzing Hsc70-4 RNA interfernce plants in an hsc70-1 mutant background. Plants with lower Hsc70 levels exhibited abnormal embryogenesis, resulting in defective seedlings that displayed high levels of reactive oxygen species and monoubiquitinated Lhcb4 precursors. We propose that Hsc70-4 and CHIP mediate plastid-destined precursor degradation to prevent cytosolic precursor accumulation and thereby play a critical role in embryogenesis.
Proteins ; Protein isoforms ; Chloroplasts ; Gels ; Reverse transcriptase polymerase chain reaction ; Protoplasts ; Antibodies ; Protein precursors ; Plastids ; Plant cells ; Oligonucleotide Array Sequence Analysis ; Plants, Genetically Modified - genetics ; HSC70 Heat-Shock Proteins - metabolism ; Ubiquitin - metabolism ; Ubiquitin-Protein Ligases - metabolism ; Phylogeny ; RNA, Plant - genetics ; Chloroplasts - metabolism ; Protein Folding ; Arabidopsis - metabolism ; Protein Precursors - metabolism ; Arabidopsis - genetics ; Arabidopsis Proteins - metabolism ; DNA, Bacterial - genetics ; Plants, Genetically Modified - metabolism ; Mutagenesis, Insertional ; Protein Processing, Post-Translational ; Proteasome Endopeptidase Complex - metabolism ; Arabidopsis thaliana ; Ubiquitin ; Heat shock proteins ; Physiological aspects ; Plant embryology ; Research ; Properties
American Society of Plant Biologists
JSTOR Life Sciences
JSTOR Ecology & Botany II
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