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  • 1
    Language: English
    In: Applied physics. A, Materials science & processing, 2020-01, Vol.126 (1)
    Description: A hybrid configuration for multicomponent thin film deposition by combining two laser ablation plasmas with one sputtering plasma is presented. This setup was used to deposit TiO2 thin films modified simultaneously with Bi and Pt. In order to investigate the effect of the metal incorporation in titania the deposited films were characterized by X-ray photoelectron, Raman, UV–Vis and photoluminescence spectroscopies, as well as by X-ray diffraction and scanning electron microscopy. The results showed that films with different load of Bi and Pt were obtained. The amount of these metals has an important effect on the compositional, structural and optical properties of the deposited films. Mixtures of TiO2 with Bi2O3 as well as metallic Bi and Pt are obtained at the highest Pt content. The index of refraction and band gap decrease as the Pt content increases making these films capable to absorb light from the visible region of the solar spectrum with potential applications as photocatalysts to degrade organic pollutants present in wastewaters.
    Subject(s): Plasma ; Bismuth trioxide ; Refractivity ; Photoluminescence ; Bismuth oxides ; Laser ablation ; Sputtering ; Thin films ; Platinum ; Optical properties ; Photoelectrons ; Plasmas ; Pollutants ; Configurations ; Titanium dioxide ; Deposition
    ISSN: 0947-8396
    E-ISSN: 1432-0630
    Source: SpringerLINK Contemporary (Konsortium Baden-Württemberg)
    Source: Academic Search Ultimate
    Source: Alma/SFX Local Collection
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 2
    Language: English
    In: Journal of applied microbiology, 2015-08, Vol.119 (2), p.365-376
    Description: Aims To produce and characterize egg yolk immunoglobulin (IgY) against the fish intracellular pathogen Piscirickettsia salmonis as well as to evaluate the antibacterial activity of IgY in vitro and the availability in the serum of fish immunized orally. Methods and Results Specific IgY was produced by immunizing hens with P. salmonis proteins. The IgY was obtained from egg yolks using the ammonium sulphate precipitation method and it was characterized by SDS‐PAGE, Western‐blot and ELISA, demonstrating that anti‐P. salmonis IgY strongly reacted specifically against P. salmonis proteins. In an in vitro neutralization assay, IgY inhibited the growth of P. salmonis in liquid medium at concentrations ranging from 128 to 256 μg ml−1 in a dose‐dependent manner. Interestingly, IgY against P. salmonis also generates a strong protective effect on the infection of P. salmonis in salmon head kidney‐1 cells. In addition, the bacteriostatic function of IgY appears to result possibly from agglutination by the interaction of IgY with surface components of the pathogen. Finally, to confirm this IgY as an alternative for salmonid treatment, Atlantic salmon (Salmo salar) specimens were orally inoculated with IgY. The analysis of the sera demonstrates that IgY was effectively transported by fish intestine and that this immunoglobulins maintains its properties and recognizes several proteins of P. salmonis up to 12 h after inoculation of IgY against P. salmonis. Conclusions Specific IgY effectively inhibited the growth of P. salmonis and this immunoglobulin can be released in the Atlantic salmon sera when administered orally to fish. Significance and Impact of the Study We propose that this specific IgY against this fastidious micro‐organism could be a useful strategy for the treatment of piscirickettsiosis.
    Subject(s): Piscirickettsia salmonis ; IgY ; growth inhibition ; immunoglobulin ; chicken egg yolk ; piscirickettsiosis ; Immunoglobulins - pharmacology ; Egg Yolk - chemistry ; Fish Diseases - microbiology ; Piscirickettsiaceae Infections - veterinary ; Electrophoresis, Polyacrylamide Gel ; Piscirickettsia - growth & development ; Piscirickettsiaceae Infections - microbiology ; Anti-Bacterial Agents - isolation & purification ; Chickens - immunology ; Piscirickettsiaceae Infections - drug therapy ; Immunoglobulins - isolation & purification ; Piscirickettsiaceae Infections - immunology ; Animals ; Fish Diseases - immunology ; Piscirickettsia - drug effects ; Anti-Bacterial Agents - pharmacology ; Fish Diseases - drug therapy ; Salmo salar - microbiology ; Index Medicus
    ISSN: 1364-5072
    E-ISSN: 1365-2672
    Source: Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
    Source: Alma/SFX Local Collection
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 3
    Language: English
    In: Journal of periodontal research, 2011-08, Vol.46 (4), p.464-474
    Description: Yokoyama M, Ukai T, Ayon Haro ER, Kishimoto T, Yoshinaga Y, Hara Y. Membrane‐bound CD40 ligand on T cells from mice injected with lipopolysaccharide accelerates lipopolysaccharide‐induced osteoclastogenesis. J Periodont Res 2011; 46: 464–474. © 2011 John Wiley & Sons A/S Background and Objective:  T cells infiltrate the inflammatory site of periodontitis and consequently stimulate the loss of periodontal bone. We previously reported that T cells from lipopolysaccharide (LPS)‐injected mice (LPS‐T cells) accelerated osteoclastogenesis in the presence of LPS. Ηowever, the detailed mechanism of this acceleration is still unclear. In this study, we analyzed the mechanism of osteoclastogenesis accelerated by LPS‐T cells. Material and Methods:  We examined the mechanism of osteoclastogenesis acceleration. First, to determine the effect of cell‐to‐cell contact, we co‐cultured T cells and bone marrow macrophages, prestimulated with RANKL for 48 h (R‐BMMs), in the presence of LPS for 24 h, in a Transwell. Second, to determine the effect of CD40 ligand (CD40L), we co‐cultured T cells and R‐BMMs in the presence of LPS and anti‐CD40L immunoglobulin. Third, we examined the effect of recombinant mouse CD40L (rCD40L) in the presence of LPS in vitro and in vivo. Lastly, we examined the expression of membrane‐bound CD40L (mCD40L) by fluorescence‐activated cell sorting (FACS). Results:  Blocking cell‐to‐cell contact between LPS‐T cells and R‐BMMs completely inhibited the acceleration of osteoclastogenesis. Anti‐CD40L immunoglobulin also completely inhibited the acceleration of osteoclastogenesis. Moreover, rCD40L accelerated osteoclastogenesis in the presence of LPS in vitro and in vivo. Finally, the expression of mCD40L on LPS‐T cells was higher than that on T cells isolated from mice not injected with LPS. Conclusion:  The results demonstrate that CD40L accelerates osteoclastogenesis in the presence of RANKL and LPS. The results also suggest that mCD40L on LPS‐T cells accelerates osteoclastogenesis.
    Subject(s): membrane‐bound CD40 ligand ; T cells ; osteoclastogenesis ; lipopolysaccharide ; Otorhinolaryngology. Stomatology ; Biological and medical sciences ; Medical sciences ; Coculture Techniques ; Escherichia coli ; Antibodies ; Recombinant Proteins ; CD40 Ligand - analysis ; RANK Ligand - pharmacology ; Flow Cytometry ; Time Factors ; T-Lymphocytes - drug effects ; Bone Marrow Cells - drug effects ; T-Lymphocytes - pathology ; Osteoclasts - pathology ; Cell Separation ; Lymphocyte Activation ; Cell Communication ; Mice, Inbred Strains ; Mice, SCID ; Alveolar Bone Loss - pathology ; Mice, Inbred ICR ; Tumor Necrosis Factor-alpha - analysis ; Animals ; CD40 Ligand - pharmacology ; Lipopolysaccharides - pharmacology ; Macrophages - drug effects ; Mice ; Osteoclasts - drug effects ; Immunoglobulins ; Mitogens ; Analysis ; Index Medicus ; Dentistry
    ISSN: 0022-3484
    E-ISSN: 1600-0765
    Source: Alma/SFX Local Collection
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 4
    Language: English
    In: Journal of periodontal research, 2011-06, Vol.46 (3), p.361-373
    Description: Ayon Haro ER, Ukai T, Yokoyama M, Kishimoto T, Yoshinaga Y, Hara Y. Locally administered interferon‐γ accelerates lipopolysaccharide‐induced osteoclastogenesis independent of immunohistological RANKL upregulation. J Periodont Res 2011; 46: 361–373. © 2011 John Wiley & Sons A/S Background and Objective:  Interferon‐γ (IFN‐γ) potently inhibits RANKL‐induced osteoclastogenesis in vitro. In contrast, previous studies have shown that an increase in IFN‐γ expression is correlated with an increase in lipopolysaccharide (LPS)‐induced bone loss in vivo. However, it is not clear whether local IFN‐γ accelerates osteoclastogenesis or not in vivo. Therefore, the aim of this study was to clarify the role of local IFN‐γ in LPS‐induced osteoclastogenesis. Material and Methods:  We induced bone loss in calvaria by injecting LPS. One group of mice received an IFN‐γ injection together with LPS injection, while another group received IFN‐γ 2 d after LPS injection. Bone resorption was observed histologically. Next, we stimulated murine bone marrow macrophages with macrophage‐colony stimulating factor and RANKL in vitro. We added different doses of IFN‐γ and/or LPS at 0 or 48 h time points. Cells were stained with tartrate‐resistant acid phosphatase at 72 h. Results:  Local administration of IFN‐γ together with LPS injection did not affect osteoclast formation. However, IFN‐γ injected after LPS injection accelerated osteoclast formation. Also, we confirmed that IFN‐γ added at 0 h inhibited RANKL‐induced osteoclastogenesis in vitro. However, inhibition by IFN‐γ added at 48 h was reduced compared with that by IFN‐γ added at 0 h. Interestingly, IFN‐γ together with a low concentration of LPS accelerated osteoclast formation when both were added at 48 h compared with no addition of IFN‐γ. Conclusion:  The results suggest that local IFN‐γ accelerates osteoclastogenesis in certain conditions of LPS‐induced inflammatory bone loss.
    Subject(s): interferon‐γ ; osteoclastogenesis ; lipopolysaccharide ; bone resorption ; Antiviral agents ; Biological and medical sciences ; Medical sciences ; Otorhinolaryngology. Stomatology ; Pharmacology. Drug treatments ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Immunohistochemistry ; Isoenzymes - analysis ; Escherichia coli ; Lipopolysaccharides - administration & dosage ; Tartrate-Resistant Acid Phosphatase ; Male ; RANK Ligand - drug effects ; RANK Ligand - pharmacology ; Time Factors ; Macrophage Colony-Stimulating Factor - pharmacology ; Bone Marrow Cells - drug effects ; Skull - drug effects ; Bone Resorption - physiopathology ; Acid Phosphatase - analysis ; Biomarkers - analysis ; Cells, Cultured ; Mice, Inbred Strains ; Up-Regulation - drug effects ; Tumor Necrosis Factor-alpha - pharmacology ; Animals ; Cell Differentiation - drug effects ; Interferon-gamma - administration & dosage ; Bone Resorption - pathology ; Lipopolysaccharides - pharmacology ; Macrophages - drug effects ; Mice ; Interferon-gamma - pharmacology ; Osteoclasts - drug effects ; Index Medicus ; Dentistry
    ISSN: 0022-3484
    E-ISSN: 1600-0765
    Source: Alma/SFX Local Collection
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 5
    Language: English
    In: International journal of photoenergy, 2018-03-19, Vol.2018, p.1-9
    Description: The synthesis of Bi-modified TiO2 thin films, with different Bi contents, is reported. The obtained materials were characterized by energy-dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy (RS), X-ray diffraction (XRD), photoluminescence (PL), and diffuse reflectance spectroscopy (DRS), in order to obtain information on their chemical composition, vibrational features, and optical properties, respectively. Compositional characterization reveals that the bismuth content can be varied in an easy way from 0.5 to 25.4 at. %. Raman results show that the starting material corresponds to the anatase phase of crystalline TiO2, and Bi addition promotes the formation of bismuth titanates, Bi2Ti2O7 at Bi contents of 10.4 at. % and the Bi4Ti3O12 at Bi contents of 21.5 and 25.4 at. %. Optical measurements reveal that the band gap narrows from 3.3 eV to values as low as 2.7 eV. The photocatalytic activity was tested in the degradation reaction of the Malachite Green carbinol base dye (MG) as a model molecule under simulated sunlight, where the most relevant result is that photocatalytic formulations containing bismuth showed higher catalytic activity than pure TiO2. The higher photocatalytic activity of MG degradation of 67% reached by the photocatalytic formulation of 21.5 at. % of bismuth is attributed to the presence of the crystalline phase perovskite-type bismuth titanate, Bi4Ti3O12.
    ISSN: 1110-662X
    E-ISSN: 1687-529X
    Source: Academic Search Ultimate
    Source: Directory of Open Access Journals
    Source: Alma/SFX Local Collection
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  • 6
    Language: English
    In: PRIZREN SOCIAL SCIENCE JOURNAL, 2020-12-31, Vol.4 (3), p.33-38
    Description: Environmental noise is a major problem with increasing presence in cities; its effects are not only assimilated into the environment, but also impact on human health. Noise sources are part of the city's daily life and it has not yet been given due attention, although prolonged exposure causes often irreversible physiological and psychosomatic damage. This paper presents a theoretical approach from several authors that expose the different tools that can be used the measurement and evaluation of environmental noise. These authors, to explain the causes and effect of environmental noise in cities consider various variables such as: sound pressure levels, weather conditions and urban structure.
    Subject(s): city, pollution, map, environmental noise
    ISSN: 2616-387X
    E-ISSN: 2616-387X
    Source: Directory of Open Access Journals
    Source: ProQuest Central
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  • 7
    Language: English
    In: Applied physics. A, Materials science & processing, 2018-02, Vol.124 (2), p.1-7
    Description: The ablation of a carbon target immersed in deionized water, in absence and in presence of ultrasonic waves is reported, and the differences investigated. The obtained nanostructures are characterized by transmission electron microscopy, Raman spectroscopy and photoluminescence. Transmission electron images reveal that the produced carbon nanostructures, with and without ultrasonic excitation, are graphene-like sheets with improved quality in the first case. Samples prepared with ultrasounds show graphene layers with large sizes (several microns) and regular shapes, whereas the samples prepared without ultrasounds show smaller sizes and irregular shapes; additionally, some dispersed quasi-spherical nanoparticles are observed in the samples prepared without ultrasound. Photoluminescence measurements of the obtained nanostructures reveal emission in the blue spectral region.
    Subject(s): Condensed Matter Physics ; Operating Procedures, Materials Treatment ; Optical and Electronic Materials ; Characterization and Evaluation of Materials ; Surfaces and Interfaces, Thin Films ; Physics ; Nanotechnology ; Raman spectroscopy ; Graphene ; Ultrasonic waves ; Graphite ; Photoluminescence ; Spectral emissivity ; Ultrasonic imaging ; Nanostructure ; Image transmission ; Laser ablation ; Electron images ; Electron microscopy ; Carbon ; Deionization
    ISSN: 0947-8396
    E-ISSN: 1432-0630
    Source: SpringerLINK Contemporary (Konsortium Baden-Württemberg)
    Source: Academic Search Ultimate
    Source: Alma/SFX Local Collection
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 8
    Language: English
    In: IEEE transactions on applied superconductivity, 2016-04, Vol.26 (3), p.1-7
    Description: EuCARD-2 is a project partly supported by FP7 European Commission aiming at exploring accelerator magnet technology for 20-T dipole operating field. The EuCARD-2 collaboration is liaising with similar programs for high-field magnets in the U.S. and Japan. EuCARD-2 focuses, through the work package 10 "future magnets," on the development of a 10-kA-class superconducting high-current-density cable suitable for accelerator magnets, for a 5-T stand-alone dipole of 40-mm bore and about 1-m length. After stand-alone testing, the magnet will be inserted in a large bore background dipole, 10-18 T. This paper reports on the design and development of models, which are called Feather0, wound with REBCO Roebel cable. Based on aligned block design to take advantage of the anisotropy of the REBCO tapes, Feather0 is a precursor of Feather2, which should reach the project goals in 2016. Feather0 is planned to be tested both in stand alone and as an insert mounted in the CERN Fresca facility providing 10-T background field. The progress of other designs pursued in the collaboration, one based on classical ϑ layout with Roebel cable and the other based on coil block with stacked tape cable, will be also reported.
    Subject(s): Superconducting cables ; Accelerators Magnets ; Power cables ; Magnetomechanical effects ; Windings ; Future Magnets ; Superconducting magnets ; High-Temperature-Superconductors ; EuCARD-2 ; Perpendicular magnetic anisotropy ; CLIQ ; Condensed Matter Physics ; Electrical and Electronic Engineering ; Electronic, Optical and Magnetic Materials ; future magnets ; Accelerators magnets ; higherature-superconductors ; superconducting magnets ; Engineering Sciences ; Electric power
    ISSN: 1051-8223
    E-ISSN: 1558-2515
    Source: IEEE Electronic Library (IEL)
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  • 9
    Language: English
    In: FEMS microbiology letters, 2016-06-01, Vol.363 (11)
    Description: Piscirickettsia salmonis is a fastidious intracellular pathogen responsible for high mortality rates in farmed salmonids, with serious economic consequences for the Chilean aquaculture industry. Oxytetracycline and florfenicol are the most frequently used antibiotics against P. salmonis, but routine use could contribute to drug resistance. This study identified differentiated florfenicol susceptibilities in two P. salmonis strains, LF-89 and AUSTRAL-005. The less susceptible isolate, AUSTRAL-005, also showed a high ethidium bromide efflux rate, indicating a higher activity of general efflux pump genes than LF-89. The P. salmonis genome presented resistance nodulation division (RND) family members, a family containing typical multidrug resistance-related efflux pumps in Gram-negative bacteria. Additionally, efflux pump acrAB genes were overexpressed in AUSTRAL-005 following exposure to the tolerated maximal concentration of florfenicol, in contrast to LF-89. These results indicate that tolerated maximum concentrations of florfenicol can modulate RND gene expression and increase efflux pump activity. We propose that the acrAB efflux pump is essential for P. salmonis survival at critical florfenicol concentrations and for the generation of antibiotic-resistant bacterial strains. Florfenicol modulates the RND efflux pump acrAB expression and contributes to drug resistance in the fish pathogen Piscirickettsia salmonis.
    Subject(s): Genome, Bacterial ; Thiamphenicol - pharmacology ; Genes, MDR ; Thiamphenicol - analogs & derivatives ; Bacterial Proteins - genetics ; Ethidium - metabolism ; Fishes - microbiology ; Microbial Sensitivity Tests ; Animals ; Membrane Transport Proteins - genetics ; Piscirickettsia - genetics ; Piscirickettsia - drug effects ; Bacterial Proteins - metabolism ; Membrane Transport Proteins - metabolism ; Anti-Bacterial Agents - pharmacology ; Chile ; Drug Resistance, Multiple, Bacterial - genetics ; Aquaculture ; Piscirickettsia - pathogenicity ; Index Medicus
    E-ISSN: 1574-6968
    Source: Oxford Journals 2016 Current and Archive A-Z Collection
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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  • 10
    Language: English
    In: The Journal of physiology, 2013-09, Vol.591 (17), p.4273-4286
    Description: Key points •  Choline (Ch) is a precursor and metabolite of the neurotransmitter acetylcholine (ACh). •  Previously, in cardiomyocytes Ch was shown to activate an outward K+ current in a delayed rectifier fashion, which has been suggested to modulate cardiac electrical activity and to play a role in atrial fibrillation pathophysiology. However, the identity of this current remains elusive. •  Single‐channel recordings, biophysical profiles and specific pharmacological inhibition indicate that the current activated by Ch is the ACh‐activated K+ current (IKACh). •  Membrane depolarization increased the potency and efficacy of IKACh activation by Ch and thus gives the appearance of a delayed rectifier activating K+ current at depolarized potentials. •  Our findings support the emerging concept that IKACh modulation is both voltage‐ and ligand‐specific and reinforce the importance of these properties in understanding cardiac physiology.   Choline (Ch) is a precursor and metabolite of the neurotransmitter acetylcholine (ACh). In canine and guinea pig atrial myocytes, Ch was shown to activate an outward K+ current in a delayed rectifier fashion. This current has been suggested to modulate cardiac electrical activity and to play a role in atrial fibrillation pathophysiology. However, the exact nature and identity of this current has not been convincingly established. We recently described the unique ligand‐ and voltage‐dependent properties of muscarinic activation of ACh‐activated K+ current (IKACh) and showed that, in contrast to ACh, pilocarpine induces a current with delayed rectifier‐like properties with membrane depolarization. Here, we tested the hypothesis that Ch activates IKACh in feline atrial myocytes in a voltage‐dependent manner similar to pilocarpine. Single‐channel recordings, biophysical profiles, specific pharmacological inhibition and computational data indicate that the current activated by Ch is IKACh. Moreover, we show that membrane depolarization increases the potency and efficacy of IKACh activation by Ch and thus gives the appearance of a delayed rectifier activating K+ current at depolarized potentials. Our findings support the emerging concept that IKACh modulation is both voltage‐ and ligand‐specific and reinforce the importance of these properties in understanding cardiac physiology.
    Subject(s): Cats ; Heart Atria - cytology ; Receptor, Muscarinic M2 - metabolism ; Male ; Delayed Rectifier Potassium Channels - metabolism ; Heart Atria - metabolism ; Action Potentials ; Animals ; Myocytes, Cardiac - drug effects ; Membrane Potentials ; Myocytes, Cardiac - physiology ; Pilocarpine - pharmacology ; Choline - pharmacology ; Myocytes, Cardiac - metabolism ; Female ; Choline ; Index Medicus ; Research Papers
    ISSN: 0022-3751
    E-ISSN: 1469-7793
    Source: Hellenic Academic Libraries Link
    Source: PubMed Central
    Source: © ProQuest LLC All rights reserved〈img src="https://exlibris-pub.s3.amazonaws.com/PQ_Logo.jpg" style="vertical-align:middle;margin-left:7px"〉
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