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  • 1
    Article
    Article
    2013
    ISSN: 0015-0282 
    Language: English
    In: Fertility and sterility, 2013, Vol.99 (3), p.616-623
    Description: In this systematic review of ovulation induction and epigenetic control, studies mainly done in the mouse model highlight how hormone treatments may be prejudicial to the epigenetic reprogramming of gametes as well as early embryos. Moreover, the hormone protocols used in assisted reproduction may also modify the physiologic environment of the uterus, a potential link to endometrial epigenetic disturbances. At present, the few available data in humans are insufficient to allow us to independently determine the impact of a woman's age and infertility problems and treatment protocols and hormone doses on such processes as genomic imprinting.
    Subject(s): Internal Medicine ; Obstetrics and Gynecology ; Epigenetics ; DNA methylation ; genomic imprinting ; ovulation induction ; ovarian stimulation ; Epigenesis, Genetic - physiology ; Reproductive Techniques, Assisted - adverse effects ; Animals ; Genetic Diseases, Inborn - prevention & control ; Ovulation Induction - methods ; Humans ; Genetic Diseases, Inborn - genetics ; Female ; Models, Animal ; Mice ; Ovulation Induction - adverse effects ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 2
    Language: English
    In: Nature (London), 2011, Vol.472 (7343), p.370-374
    Description: X-chromosome inactivation (XCI) in female mammals allows dosage compensation for X-linked gene products between the sexes. The developmental regulation of this process has been extensively investigated in mice, where the X chromosome of paternal origin (Xp) is silenced during early embryogenesis owing to imprinted expression of the regulatory RNA, Xist (X-inactive specific transcript). Paternal XCI is reversed in the inner cell mass of the blastocyst and random XCI subsequently occurs in epiblast cells. Here we show that other eutherian mammals have very different strategies for initiating XCI. In rabbits and humans, the Xist homologue is not subject to imprinting and XCI begins later than in mice. Furthermore, Xist is upregulated on both X chromosomes in a high proportion of rabbit and human embryo cells, even in the inner cell mass. In rabbits, this triggers XCI on both X chromosomes in some cells. In humans, chromosome-wide XCI has not initiated even by the blastocyst stage, despite the upregulation of XIST. The choice of which X chromosome will finally become inactive thus occurs downstream of Xist upregulation in both rabbits and humans, unlike in mice. Our study demonstrates the remarkable diversity in XCI regulation and highlights differences between mammals in their requirement for dosage compensation during early embryogenesis.
    Subject(s): Fundamental and applied biological sciences. Psychology ; Biological and medical sciences ; Cell physiology ; Molecular and cellular biology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Mammals - genetics ; X Chromosome Inactivation - genetics ; Species Specificity ; Chromosomes, Mammalian - genetics ; Humans ; Gene Expression Regulation, Developmental - genetics ; Dosage Compensation, Genetic - genetics ; Male ; RNA, Untranslated - genetics ; Embryo, Mammalian - metabolism ; Mammals - embryology ; Female ; X Chromosome - genetics ; Parthenogenesis ; Rabbits ; Genes, X-Linked - genetics ; Blastocyst - metabolism ; Up-Regulation - genetics ; Biological Evolution ; Genomic Imprinting - genetics ; Animals ; Embryo, Mammalian - embryology ; Hypoxanthine Phosphoribosyltransferase - genetics ; RNA, Long Noncoding ; Mice ; Histones - metabolism ; Index Medicus ; Life Sciences ; Development Biology ; Reproductive Biology
    ISSN: 0028-0836
    E-ISSN: 1476-4687
    E-ISSN: 1476-4679
    Source: Academic Search Ultimate
    Source: Alma/SFX Local Collection
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  • 3
    Language: English
    In: BMC developmental biology, 2007-10-18, Vol.7 (1), p.116-116
    Description: In the last few years, an increase in imprinting anomalies has been reported in children born from Assisted Reproductive Technology (ART). Various clinical and experimental studies also suggest alterations of embryo development after ART. Therefore, there is a need for studying early epigenetic anomalies which could result from ART manipulations, especially on single embryos. In this study, we evaluated the impact of superovulation, in vitro fertilization (IVF) and embryo culture conditions on proper genomic imprinting and blastocyst development in single mouse embryos. In this study, different experimental groups were established to obtain embryos from superovulated and non-superovulated females, either from in vivo or in vitro fertilized oocytes, themselves grown in vitro or not. The embryos were cultured either in M16 medium or in G1.2/G2.2 sequential medium. The methylation status of H19 Imprinting Control Region (ICR) and H19 promoter was assessed, as well as the gene expression level of H19, in individual blastocysts. In parallel, we have evaluated embryo cleavage kinetics and recorded morphological data. We show that: 1. The culture medium influences early embryo development with faster cleavage kinetics for culture in G1.2/G2.2 medium compared to M16 medium. 2. Epigenetic alterations of the H19 ICR and H19 PP are influenced by the fertilization method since methylation anomalies were observed only in the in vitro fertilized subgroup, however to different degrees according to the culture medium. 3. Superovulation clearly disrupted H19 gene expression in individual blastocysts. Moreover, when embryos were cultured in vitro after either in vivo or in vitro fertilization, the percentage of blastocysts which expressed H19 was higher in G1.2/G2.2 medium compared to M16. Compared to previous reports utilizing pools of embryos, our study enables us to emphasize a high individual variability of blastocysts in the H19 ICR and H19 promoter methylation and H19 gene expression, with a striking effect of each manipulation associated to ART practices. Our results suggest that H19 could be used as a sensor of the epigenetic disturbance of the utilized techniques.
    Subject(s): Fertilization in Vitro - adverse effects ; Genomic Imprinting ; Epigenesis, Genetic ; Superovulation ; Blastocyst - metabolism ; Male ; RNA, Untranslated - genetics ; Embryo, Mammalian - metabolism ; DNA Methylation ; Animals ; Embryo, Mammalian - embryology ; Gene Expression Regulation, Developmental ; RNA, Long Noncoding ; Female ; Mice ; Embryology ; Research ; Gene expression ; Health aspects ; Reproductive technology ; Index Medicus ; Life Sciences ; Computer Science
    ISSN: 1471-213X
    E-ISSN: 1471-213X
    Source: BioMedCentral Open Access
    Source: Academic Search Ultimate
    Source: PubMed Central
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  • 4
    Language: English
    In: Cellular and molecular life sciences : CMLS, 2020-02, Vol.77 (3), p.511-529
    Description: The sperm acrosome is a lysosome-related organelle that develops using membrane trafficking from the Golgi apparatus as well as the endolysosomal compartment. How vesicular trafficking is regulated in spermatids to form the acrosome remains to be elucidated. VPS13B, a RAB6-interactor, was recently shown involved in endomembrane trafficking. Here, we report the generation of the first Vps13b-knockout mouse model and show that male mutant mice are infertile due to oligoasthenoteratozoospermia. This phenotype was explained by a failure of Vps13b deficient spermatids to form an acrosome. In wild-type spermatids, immunostaining of Vps13b and Rab6 revealed that they transiently locate to the acrosomal inner membrane. Spermatids lacking Vps13b did not present with the Golgi structure that characterizes wild-type spermatids and showed abnormal targeting of PNA- and Rab6-positive Golgi-derived vesicles to Eea1- and Lamp2-positive structures. Altogether, our results uncover a function of Vps13b in the regulation of the vesicular transport between Golgi apparatus, acrosome, and endolysosome.
    Subject(s): Spermatids - metabolism ; Acrosome - metabolism ; Vesicular Transport Proteins - metabolism ; Male ; Protein Transport - physiology ; Spermatozoa - metabolism ; Mice, Knockout ; Spermatogenesis - physiology ; Animals ; Lysosomes - metabolism ; Golgi Apparatus - metabolism ; Biological Transport - physiology ; Mice ; Analysis ; Biosynthesis ; Index Medicus
    ISSN: 1420-682X
    E-ISSN: 1420-9071
    Source: Alma/SFX Local Collection
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  • 5
    Language: English
    In: European journal of human genetics : EJHG, 2010, Vol.18 (1), p.73-80
    Description: DNA methylation marks, a key modification of imprinting, are erased in primordial germ cells and sex specifically re-established during gametogenesis. Abnormal epigenetic programming has been proposed as a possible mechanism compromising male fertility. We analysed by pyrosequencing the DNA methylation status of 47 CpGs located in differentially methylated regions (DMRs), the DMR0 and DMR2 of the IGF2 gene and in the 3rd and 6th CTCF-binding sites of the H19 DMR in human sperm from men with normal semen and patients with teratozoospermia (T) and/or oligo-astheno-teratozoospermia (OAT). All normal semen samples presented the expected high global methylation level for all CpGs analysed. In the teratozoospermia group, 11 of 19 patients presented a loss of methylation at variable CpG positions either in the IGF2 DMR2 or in both the IGF2 DMR2 and the 6th CTCF of the H19 DMR. In the OAT group, 16 of 22 patients presented a severe loss of methylation of the 6th CTCF, closely correlated with sperm concentration. The methylation state of DMR0 and of the 3rd CTCF was never affected by the pathological status of sperm samples. This study demonstrates that epigenetic perturbations of the 6th CTCF site of the H19 DMR might be a relevant biomarker for quantitative defects of spermatogenesis in humans. Moreover, we defined a methylation threshold sustaining the classification of patients in two groups, unmethylated and methylated. Using this new classification of patients, the observed intrinsic imprinting defects of spermatozoa appear not to impair significantly the outcome of assisted reproductive technologies.
    Subject(s): Fundamental and applied biological sciences. Psychology ; Classical genetics, quantitative genetics, hybrids ; Human ; General aspects. Genetic counseling ; Medical genetics ; Biological and medical sciences ; Molecular and cellular biology ; Genetics of eukaryotes. Biological and molecular evolution ; Medical sciences ; Spermatozoa - pathology ; Epigenesis, Genetic ; Humans ; Genetic Loci - genetics ; Male ; Treatment Outcome ; DNA Methylation - genetics ; Spermatozoa - metabolism ; Infertility, Male - pathology ; Reproductive Techniques, Assisted ; Infertility, Male - genetics ; Insulin-Like Growth Factor II - genetics ; CpG Islands - genetics ; Adult ; Binding Sites ; Cohort Studies ; Life Sciences ; Computer Science ; pyrosequencing ; IGF2-H19 locus ; genomic imprinting ; spermatozoa ; methylation ; assisted reproductive technologies (ART)
    ISSN: 1018-4813
    E-ISSN: 1476-5438
    Source: Nature Open Access
    Source: Academic Search Ultimate
    Source: PubMed Central
    Source: Alma/SFX Local Collection
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  • 6
    Language: English
    In: Scientific reports, 2018-02-02, Vol.8 (1), p.2202-13
    Description: Progress in assisted reproductive technologies strongly relies on understanding the regulation of the dialogue between oocyte and cumulus cells (CCs). Little is known about the role of long non-coding RNAs (lncRNAs) in the human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that were abundant in metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, and LINC01118) and CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, and VIM-AS1). These data were validated by RT-qPCR analysis using independent oocytes and CC samples. The functions of the identified lncRNAs were then predicted by constructing lncRNA-mRNA co-expression networks. This analysis suggested that MII oocyte lncRNAs could be involved in chromatin remodeling, cell pluripotency and in driving early embryonic development. CC lncRNAs were co-expressed with genes involved in apoptosis and extracellular matrix-related functions. A bioinformatic analysis of RNA-sequencing data to identify CC lncRNAs that are affected by maternal age showed that lncRNAs with age-related altered expression in CCs are essential for oocyte growth. This comprehensive analysis of lncRNAs expressed in human MII oocytes and CCs could provide biomarkers of oocyte quality for the development of non-invasive tests to identify embryos with high developmental potential.
    Subject(s): RNA, Long Noncoding - analysis ; Oocytes - physiology ; Humans ; Metaphase ; Computational Biology ; Gene Expression Profiling ; Reverse Transcriptase Polymerase Chain Reaction ; Real-Time Polymerase Chain Reaction ; Cumulus Cells - physiology ; Data processing ; mRNA ; Gene expression ; Oocytes ; Chromatin remodeling ; Interleukin 6 ; Reproduction ; Embryogenesis ; Extracellular matrix ; Non-coding RNA ; Pluripotency ; Age ; Apoptosis ; Index Medicus ; Life Sciences
    ISSN: 2045-2322
    E-ISSN: 2045-2322
    Source: Nature Open Access
    Source: Academic Search Ultimate
    Source: PubMed Central
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  • 7
    Language: English
    In: Fertility and sterility, 2016, Vol.107 (1), p.97-103.e4
    Description: Objective To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. Design Retrospective study. Setting Hospital. Patient(s) Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November 2012 to December 2014 were included. A total of 2,449 embryos were assessed for multinucleation rates at the two- and four-cell stage, and 608 transferred embryos were studied for ICSI outcomes. Intervention(s) None. Main Outcome Measure(s) Implantation rate (IR) and live birth rate (LBR) according to the number of multinucleated blastomeres at the two-cell stage: none (Without-MNB2cell ), one (MNB1/2cell ), and two (MNB2/2cell ); morphokinetics of MNB2cell embryos. Result(s) Embryos with MNB1/2cell led to lower IR (27.7%) and LBR (22.7%) than embryos Without-MNB2cell (33.4% and 29.8%, respectively). The MNB2/2cell embryos led to significantly lower IR (18.3%) and LBR (13.4%) than embryos Without-MNB2cell . This difference remained significant in multivariate analysis for implantation (odds ratio 0.57; 95% confidence interval 0.34–0.94) and birth (odds ratio 0.46; 95% confidence interval 0.26–0.80), independently of the other significant parameters (women's age, time of two-cell formation, and multinucleation at the four-cell stage). Among implanted MNB2cell , if cleavage into four cells occurred later than 37 hours after insemination, embryos were significantly more likely to lead to birth. Conclusion(s) The presence of multinucleation at the two-cell stage and more specifically in both blastomeres had a significant negative impact on birth potential. Thus, embryo multinucleation at the two-cell stage should be used as an additional noninvasive criterion for embryo selection.
    Subject(s): Internal Medicine ; Obstetrics and Gynecology ; Birth rate ; implantation ; ICSI outcomes ; embryo multinucleation ; time-lapse system ; Multivariate Analysis ; Humans ; Microscopy, Video ; Cleavage Stage, Ovum ; Embryo Transfer - adverse effects ; Fertility ; Female ; Infertility - physiopathology ; Time-Lapse Imaging - methods ; Retrospective Studies ; Odds Ratio ; Infertility - therapy ; Pregnancy Rate ; Live Birth ; Risk Factors ; Logistic Models ; Treatment Outcome ; Blastomeres - cytology ; Chi-Square Distribution ; Embryo Implantation ; Pregnancy ; Infertility - diagnosis ; Embryo, Mammalian - cytology ; Sperm Injections, Intracytoplasmic - adverse effects ; Cell Nucleus ; Embryonic development ; Spermatozoa ; Embryo ; Analysis ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 8
    Language: English
    In: Fertility and sterility, 2010, Vol.94 (7), p.2796-2799
    Description: In women with endometriosis, including those with endometriomas, 6 to 8 weeks of continuous use of oral contraception (OC) before assisted reproduction treatment (ART) maintains ART outcomes comparable with the outcomes of age-matched controls without endometriosis. In contrast, ART outcomes are markedly compromised in endometriosis patients who are not pretreated with OC. Ovarian responsiveness to stimulation was not altered by 6 to 8 weeks' use of pre-ART OC, including in poor responders with endometriomas.
    Subject(s): Internal Medicine ; Obstetrics and Gynecology ; oral contraceptives ; ART ; endometriosis ; IVF ; endometriomas ; controlled ovarian hyperstimulation ; COH ; Gynecology. Andrology. Obstetrics ; Birth control ; Non tumoral diseases ; Biological and medical sciences ; Medical sciences ; Sterility. Assisted procreation ; Female genital diseases ; Prognosis ; Drug Administration Schedule ; Humans ; Uterine Diseases - therapy ; Treatment Outcome ; Combined Modality Therapy ; Triptorelin Pamoate - administration & dosage ; Endometriosis - complications ; Reproductive Techniques, Assisted ; Uterine Diseases - complications ; Pregnancy ; Endometriosis - therapy ; Ovulation Induction - methods ; Contraceptives, Oral - administration & dosage ; Infertility, Female - etiology ; Adult ; Female ; Gonadotropin-Releasing Hormone - agonists ; Infertility, Female - diagnosis ; Infertility, Female - therapy ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 9
    Language: English
    In: Fertility and sterility, 2014, Vol.101 (6), p.1618-1623.e3
    Description: Objective To determine the impact of the time interval from the end of sperm preparation (TSP) to intrauterine insemination (IUI) on the outcome. Design Prospective multicentre cohort study. Setting Seven French centers (assisted reproduction group in northeastern France, four academic centers, and three clinics). Patient(s) Eight hundred sixty-two IUI cycles (709 patients) managed by gonadotropins were studied. Intervention(s) Cycles were stimulated by either FSH or hMG, and hCG was administrated when the leading follicle diameter measured 〉15 mm. IUIs were performed ∼36 hours after ovulation triggering. Main Outcome Measure(s) Generalized linear mixed models for binary outcomes were used to model clinical pregnancy (CP) to assess the effect of TSP adjusted for other predictors (such as maternal age, semen quality, and indication of IUI treatment). Result(s) The TSP effect was significant, featuring an inverse U-shaped curve admitting an optimum interval of ∼40–80 minutes improving CP compared with other values. Other significant predictors were total motile spermatozoa inseminated, maternal age, and unexplained infertility. Conclusion(s) The observance of TSP in the range of 40–80 minutes has a potential positive effect on pregnancy rate, while not requiring the investment of supplemental resources. This finding awaits confirmation in randomized trials.
    Subject(s): Internal Medicine ; Obstetrics and Gynecology ; sperm process ; Intrauterine insemination ; timing ; pregnancy ; Multivariate Analysis ; Prospective Studies ; Age Factors ; Ovulation Induction ; Humans ; Male ; Time Factors ; Fertility ; Adult ; Female ; Infertility - physiopathology ; France ; Infertility - etiology ; Odds Ratio ; Fertility Agents, Female - administration & dosage ; Infertility - therapy ; Pregnancy Rate ; Spermatozoa - pathology ; Semen Analysis ; Specimen Handling - methods ; Linear Models ; Treatment Outcome ; Insemination, Artificial, Homologous ; Pregnancy ; Tissue Donors ; Spermatozoa ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 10
    Language: English
    In: Fertility and sterility, 2007, Vol.87 (5), p.1200-1207
    Description: Objective To check the efficiency of the cryopreservation procedure by using trypan blue staining of ovarian test fragments to assess the quality of frozen–thawed ovarian tissue. Design Prospective study. Setting University hospital. Patient(s) Patients with polycystic ovary syndrome undergoing laparoscopic ovarian drilling. Intervention(s) Ovarian cortical biopsies obtained from polycystic ovary syndrome patients were frozen using a slow freezing–rapid thawing protocol. Main Outcome Measure(s) Primordial and primary follicle viability was assessed with trypan blue staining. Unstained isolated follicles were studied by transmission electron microscopy. Histologic and immunohistochemical analysis of apoptosis was performed on tissue sections. Result(s) The percentage of unstained follicles considered live was lower ( P =.015) after freezing/thawing (71.9%) than before cryopreservation (87.3%). Transmission electron microscopy on follicles considered live confirmed the lack of ultrastructural damage. After freezing/thawing, tissue morphology was preserved, but immunohistochemical analysis shows a significant increase in the apoptosis process. Conclusion(s) Ovarian cortical test fragments combined with trypan blue staining on enzymatically isolated follicles is a useful and quick method of assessing the initial quality and viability of follicles in cryopreserved ovarian tissue. This type of test should be used routinely as quality control in ovarian cryopreservation procedures.
    Subject(s): Internal Medicine ; Obstetrics and Gynecology ; ovarian tissue ; cryopreservation ; Apoptosis ; viability ; Gynecology. Andrology. Obstetrics ; Biological and medical sciences ; Medical sciences ; Staining and Labeling - methods ; Prospective Studies ; Ovary - chemistry ; Staining and Labeling - standards ; Humans ; Trypan Blue - analysis ; Cryopreservation - methods ; Cryopreservation - standards ; Cryoprotective Agents - analysis ; Adult ; Female ; Ovary - ultrastructure ; Polycystic Ovary Syndrome - pathology ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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