OncoTargets and therapy, 2016, Vol.9, p.7451-7458
Nowadays, despite great progress in cancer research, the detailed mechanisms of colorectal cancer (CRC) are still poorly understood. Circular RNAs (circRNAs), a new star of the non-coding RNA network, have been identified as critical regulators in various cancers, including CRC.
In this study, by using unsupervised hierarchical clustering analysis, a novel dysregulated circRNA, hsa_circ_0000069, was found. The expression of hsa_circ_0000069 was measured in 30 paired CRC tissues and adjacent noncancerous tissues using quantitative polymerase chain reaction. A high expression of hsa_circ_0000069 was observed in CRC tissues and correlated with patients' age and tumor, node, metastasis (TNM) stage (
〈0.05). Furthermore, by using specifically designed siRNAs in CRC cells, a functional analysis was performed which revealed that hsa_circ_0000069 knockdown could notably inhibit cell proliferation, migration, and invasion, and induce G0/G1 phase arrest of cell cycle in vitro.
This study's findings are the first to demonstrate that hsa_circ_0000069, an important regulator in cancer progression, could be a promising target in the diagnosis and therapy in colorectal cancer.
Cell proliferation ; Prognosis ; MicroRNA ; Analysis ; Colorectal cancer ; Development and progression ; Diagnosis ; Research ; Biological markers ; Care and treatment ; Genetic aspects ; Gene expression ; Health aspects ; Oncogenes ; Data analysis ; Dehydrogenases ; Clustering ; Stomach cancer ; Cell adhesion & migration ; Polymerase chain reaction ; Liver cancer ; Cell growth ; Hospitals ; MicroRNAs ; Cell cycle ; Biomarkers ; Alzheimers disease ; Apoptosis ; hsa_circ_0000069 ; colorectal cancer ; regulation ; Original Research ; circular RNA
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