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  • 1
    Article
    Article
    2007
    ISSN: 1466-5026 
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2007-03-01, Vol.57 (3), p.572-576
    Description: 1 Institut für Mikrobiologie, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany 2 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Wien, Austria 3 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany Correspondence Hans-Jürgen Busse Hans-Juergen.Busse{at}vu-wien.ac.at The taxonomic position of Pseudomonas sp. B13 T , isolated as a 3-chlorobenzoate-degrading organism and used for several groundbreaking studies on the enzymology and genetics of the degradative pathway for haloaromatic compounds, was studied in detail. The previously performed physiological studies, the detection of ubiquinone Q-9, the polyamine pattern with putrescine and spermidine as major polyamines, a fatty acid profile with C 18 : 1 7 c , summed feature 3 and C 16 : 0 as quantitatively the most important constituents and the 16S rRNA gene sequence demonstrated that Pseudomonas sp. B13 T indeed belongs to the genus Pseudomonas . The sequence of the Pseudomonas sp. B13 T 16S rRNA gene demonstrated a high degree of similarity with that of Pseudomonas citronellolis DSM 50332 T (98.9 %), Pseudomonas nitroreducens DSM 14399 T (98.7 %), Pseudomonas jinjuensis DSM 16612 T (98.1 %) and Pseudomonas multiresinivorans DSM 17553 T (98.7 %). Thus it was shown that strain Pseudomonas sp. B13 T can be distinguished from related species by the ability/inability to assimilate N -acetylgalactosamine, D -galactose, putrescine, trans -aconitate and mesaconate and some differences in the fatty acid profile. The positioning of Pseudomonas sp. B13 T as a separate taxon was finally verified by DNA hybridization, which demonstrated less than 45 % DNA–DNA similarity between strain Pseudomonas sp. B13 T and the reference strains. On the basis of these results, Pseudomonas sp. B13 T represents a novel species for which the name Pseudomonas knackmussii sp. nov. is proposed. The type strain is B13 T (=DSM 6978 T =LMG 23759 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Pseudomonas sp. B13 T is AF039489 .
    Subject(s): Bacteriology ; Biological and medical sciences ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Fatty Acids - analysis ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Molecular Sequence Data ; Pseudomonas - chemistry ; Pseudomonas - classification ; Pseudomonas - genetics ; Pseudomonas - physiology ; RNA, Ribosomal, 16S - genetics ; Systematics
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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  • 2
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2010-04, Vol.60 (Pt 4), p.801-808
    Description: A Gram-negative, non-motile, non-spore-forming coccoid bacterium (strain BO1(T)) was isolated recently from a breast implant infection of a 71-year-old female patient with clinical signs of brucellosis. Affiliation of strain BO1(T) to the genus Brucella was confirmed by means of polyamine pattern, polar lipid profile, fatty acid profile, quinone system, DNA-DNA hybridization studies and by insertion sequence 711 (IS711)-specific PCR. Strain BO1(T) harboured four to five copies of the Brucella-specific insertion element IS 711, displaying a unique banding pattern, and exhibited a unique 16S rRNA gene sequence and also grouped separately in multilocus sequence typing analysis. Strain BO1(T) reacted with Brucella M-monospecific antiserum. Incomplete lysis was detected with bacteriophages Tb (Tbilisi), F1 and F25. Biochemical profiling revealed a high degree of enzymic activity and metabolic capabilities. In multilocus VNTR (variable-number tandem-repeat) analysis, strain BO1(T) showed a very distinctive profile and clustered with the other 'exotic' Brucella strains, including strains isolated from marine mammals, and Brucella microti, Brucella suis biovar 5 and Brucella neotomae. Comparative omp2a and omp2b gene sequence analysis revealed the most divergent omp2 sequences identified to date for a Brucella strain. The recA gene sequence of strain BO1(T) differed in seven nucleotides from the Brucella recA consensus sequence. Using the Brucella species-specific multiplex PCR assay, strain BO1(T) displayed a unique banding pattern not observed in other Brucella species. From the phenotypic and molecular analysis it became evident that strain BO1( T) was clearly different from all other Brucella species, and therefore represents a novel species within the genus Brucella. Because of its unexpected isolation, the name Brucella inopinata with the type strain BO1(T) (=BCCN 09-01(T)=CPAM 6436(T)) is proposed.
    Subject(s): Aged ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Typing Techniques ; Breast Implantation - adverse effects ; Breast Implants - microbiology ; Brucella - classification ; Brucella - genetics ; Brucella - isolation & purification ; Brucella - physiology ; Brucellosis - microbiology ; DNA, Ribosomal - analysis ; DNA, Ribosomal - genetics ; Fatty Acids - analysis ; Female ; Genes, rRNA ; Genotype ; Humans ; Life Sciences ; Minisatellite Repeats ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Phenotype ; Phylogeny ; Prosthesis-Related Infections - microbiology ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Species Specificity
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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  • 3
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2016-01, Vol.66 (1), p.9-37
    Description: In this paper, the taxonomy of the genus Arthrobacter is discussed, from its first description in 1947 to the present state. Emphasis is given to intrageneric phylogeny and chemotaxonomic characteristics, concentrating on quinone systems, peptidoglycan compositions and polar lipid profiles. Internal groups within the genus Arthrobacter indicated from homogeneous chemotaxonomic traits and corresponding to phylogenetic grouping and/or high 16S rRNA gene sequence similarities are highlighted. Furthermore, polar lipid profiles and quinone systems of selected species are shown, filling some gaps concerning these chemotaxonomic traits. Based on phylogenetic groupings, 16S rRNA gene sequence similarities and homogeneity in peptidoglycan types, quinone systems and polar lipid profiles, a description of the genus Arthrobacter sensu lato and an emended description of Arthrobacter roseus are provided. Furthermore, reclassifications of selected species of the genus Arthrobacter into novel genera are proposed, namely Glutamicibacter gen. nov. (nine species), Paeniglutamicibacter gen. nov. (six species), Pseudoglutamicibacter gen. nov. (two species), Paenarthrobacter gen. nov. (six species) and Pseudarthrobacter gen. nov. (ten species).
    Subject(s): Arthrobacter - classification ; Base Composition ; DNA, Bacterial - genetics ; Fatty Acids - chemistry ; Glycolipids - chemistry ; Peptidoglycan - chemistry ; Phospholipids - chemistry ; Phylogeny ; Quinones - chemistry ; RNA, Ribosomal, 16S - genetics ; Terminology as Topic
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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  • 4
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2012, Vol.62 (Pt 12), p.2835-2843
    Description: 'Caulobacter leidyi' DSM 4733(T) has been shown to be affiliated with the family Sphingomonadaceae instead of the Caulobacteraceae, and due to its poor characterization has been omitted from the current edition of Bergey's Manual of Systematic Bacteriology and removed to limbo. We isolated a novel sphingoglycolipid-containing dimorphic prosthecate bacterium, designated strain 247, from a pre-alpine freshwater lake. Strain 247 and 'Caulobacter leidyi' DSM 4733(T) were characterized in detail. The rod-shaped cells were Gram-stain-negative, aerobic, catalase- and oxidase-positive, and formed a stalk or polar flagellum. Both strains grew optimally at 28-30 °C, and pH 6.0-8.0. The major fatty acids were C(18 : 1)ω7c, C(16 : 0) and 11-methyl C(18 : 1)ω7c. C(14 : 0) 2-OH represents the major 2-hydroxy fatty acid. Q-10 was the major respiratory quinone and the major polar lipids were diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, three glycolipids, two phosphoaminolipids and two unidentified sphingoglycolipids. The major polyamine was sym-homospermidine. The G+C content of genomic DNA of strains 247 and DSM 4733(T) was 67.6 mol% and 67.0 mol%, respectively. According to 16S rRNA gene sequence analysis and DNA-DNA hybridization, strains DSM 4733(T) and 247 were phylogenetically closely related (99.6 % 16S rRNA gene sequence similarity, 82.9 % DNA-DNA hybridization value) and affiliated to the genus Sphingomonas. The closest recognized species was Sphingomonas aquatilis DSM 15581(T) (98.1 % sequence similarity). In addition, the presence of cystine arylamidase, absence of β-galactosidase, and the inability to utilize l-arabinose, galactose and sucrose distinguished strains DSM 4733(T) and 247 from most other members of the family Sphingomonadaceae. So far, the dimorphic life cycle that involves a prosthecate and a flagellated stage is unique for strains DSM 4733(T) and 247 among all members of the family Sphingomonadaceae. Therefore, Caulobacter leidyi is reclassified as Sphingomonas leidyi, with the type strain DSM 4733(T) ( = ATCC 15260(T) = CIP 106443(T) = VKM B-1368(T)) and strain 247 (DSM 25078 = LMG 26658) as an additional strain of this species.
    Subject(s): Bacterial Typing Techniques ; Bacteriology ; Base Composition ; Biological and medical sciences ; Caulobacter - classification ; Caulobacter - genetics ; DNA, Bacterial - genetics ; Fatty Acids - analysis ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Phylogeny ; Quinones - analysis ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Spermidine - analogs & derivatives ; Spermidine - analysis ; Sphingomonas - classification ; Sphingomonas - genetics ; Systematics
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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  • 5
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2008-02-01, Vol.58 (2), p.375-382
    Description: 1 Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany 2 Institute of Vertebrate Biology, Academy of Sciences, Kvetna 8, CZ-60365 Brno, Czech Republic 3 Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Brno, Czech Republic 4 Centre d'Etudes du Bouchet, BP3, 91710 Vert le Petit, France 5 Université Paris-Sud, Institut de Génétique et Microbiologie, Orsay, F-91405, France; CNRS, Orsay F-91405, France 6 Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, German Reference Center for Brucellosis, Jena, Germany 7 Institute for Applied Microbiology, Justus-Liebig-Universität Giessen, IFZ, Heinrich-Buff- Ring 26-32, D-35392 Giessen, Germany 8 INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly, F-37380, France 9 Veterinary Laboratories Agency, Woodham Lane, Addlestone KT15 3NB, UK 10 Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Guldhedsg. 10 S-41346 Göteborg, Sweden 11 Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D-12277 Berlin, Germany 12 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria Correspondence Holger C. Scholz Holger1Scholz{at}bundeswehr.org Two Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains CCM 4915 T and CCM 4916), isolated from clinical specimens of the common vole Microtus arvalis during an epizootic in the Czech Republic in 2001, were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA ( rrs ) and recA gene sequence similarities, both isolates were allocated to the genus Brucella . Affiliation to Brucella was confirmed by DNA–DNA hybridization studies. Both strains reacted equally with Brucella M-monospecific antiserum and were lysed by the bacteriophages Tb, Wb, F1 and F25. Biochemical profiling revealed a high degree of enzyme activity and metabolic capabilities not observed in other Brucella species. The omp2a and omp2b genes of isolates CCM 4915 T and CCM 4916 were indistinguishable. Whereas omp2a was identical to omp2a of brucellae from certain pinniped marine mammals, omp2b clustered with omp2b of terrestrial brucellae. Analysis of the bp26 gene downstream region identified strains CCM 4915 T and CCM 4916 as Brucella of terrestrial origin. Both strains harboured five to six copies of the insertion element IS 711 , displaying a unique banding pattern as determined by Southern blotting. In comparative multilocus VNTR (variable-number tandem-repeat) analysis (MLVA) with 296 different genotypes, the two isolates grouped together, but formed a separate cluster within the genus Brucella . Multilocus sequence typing (MLST) analysis using nine different loci also placed the two isolates separately from other brucellae. In the IS 711 -based AMOS PCR, a 1900 bp fragment was generated with the Brucella ovis -specific primers, revealing that the insertion element had integrated between a putative membrane protein and cboL , encoding a methyltransferase, an integration site not observed in other brucellae. Isolates CCM 4915 T and CCM 4916 could be clearly distinguished from all known Brucella species and their biovars by means of both their phenotypic and molecular properties, and therefore represent a novel species within the genus Brucella , for which the name Brucella microti sp. nov. with the type strain CCM 4915 T (=BCCN 07-01 T =CAPM 6434 T ) is proposed. Abbreviations: MLST, multilocus sequence typing; MLVA, multilocus VNTR (variable-number tandem-repeat) analysis; RTD, routine test dilution The GenBank/EMBL/DDBJ accession numbers for the gene sequences omp22 , omp25 , omp25b , omp31 and omp31b of strain CCM 4915 T are AM712379 , AM712381 , AM712383 , AM712385 and AM712387 , respectively. Present address : RWTH University of Aachen, Department of Internal Medicine III, Pauwelsstraße 30, D-52074 Aachen, Germany.
    Subject(s): Animals ; Arvicolinae - microbiology ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Typing Techniques ; Bacteriology ; Biochemistry, Molecular Biology ; Biological and medical sciences ; biotypes ; Brucella - classification ; Brucella - genetics ; Brucella - isolation & purification ; Brucella - physiology ; Brucellosis - microbiology ; Brucellosis - veterinary ; classification ; differentiation ; dna polymorphism ; DNA, Bacterial - analysis ; Fundamental and applied biological sciences. Psychology ; Genes, rRNA ; Genotype ; identification ; Life Sciences ; lipid-composition ; marine mammals ; melitensis ; Microbiology ; Microbiology and Parasitology ; Microbiology in the medical area ; Mikrobiologi inom det medicinska området ; Minisatellite Repeats - genetics ; Miscellaneous ; Molecular Sequence Data ; multiplex pcr assay ; Nucleic Acid Hybridization ; Phenotype ; Phylogeny ; Rec A Recombinases - genetics ; RNA, Ribosomal, 16S - genetics ; Rodent Diseases - microbiology ; Sequence Analysis, DNA ; Species Specificity ; strains ; Systematics
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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  • 6
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2006-04-01, Vol.56 (4), p.781-786
    Description: 1 Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, IFZ–Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany 2 Grup d'Oceanografia Interdisciplinari, Institut Mediterrani d'Estudis Avançats (CSIC-UIB), Esporles, Mallorca, Spain 3 CCUG, Culture Collection University of Göteborg, Göteborg, Sweden 4 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Wien, Austria 5 DSMZ, Mascheroder Weg 1b, D-38240 Braunschweig, Germany Correspondence Peter Kämpfer peter.kaempfer{at}agrar.uni-giessen.de A Gram-positive, rod-shaped, endospore-forming organism, strain CCUG 47242 T , was isolated from a sample of industrial starch production in Sweden. 16S rRNA gene sequence analysis demonstrated that this isolate was moderately related to species of the genus Paenibacillus , with 〈94·4 % sequence similarity to all other hitherto described Paenibacillus species. Strain CCUG 47242 T showed the greatest sequence similarity (96·5 %) to ‘ Paenibacillus hongkongensis ’ HKU3, a strain with a name that has not yet been validly published. Chemotaxonomic data [major menaquinone, MK-7 (98 %); major polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, two unknown phospholipids, four unknown aminophospholipids; major fatty acids, iso-C16 : 0 and anteiso-C15 : 0] showed some significant differences when compared with the type species of the genus Paenibacillus , Paenibacillus polymyxa . Physiological and biochemical tests allowed clear phenotypic differentiation of strain CCUG 47242 T from strain HKU3. On the basis of 16S rRNA gene sequence analysis, in combination with chemotaxonomic data, strains CCUG 47242 T and HKU3 represent two novel species of a new genus of endospore-forming bacteria for which we propose the names Cohnella thermotolerans gen. nov., sp. nov. (type strain CCUG 47242 T =CIP 108492 T =DSM 17683 T ) and Cohnella hongkongensis sp. nov. (type strain HKU3 T =CCUG 49571 T =CIP 107898 T =DSM 17642 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of CCUG 47242 T is AJ971483 . Phylogenetic trees constructed using the neighbour-joining and maximum-parsimony methods are available as supplementary figures in IJSEM Online.
    Subject(s): Bacteriology ; Biological and medical sciences ; emended description ; Fatty Acids - analysis ; Fundamental and applied biological sciences. Psychology ; Gram-Positive Endospore-Forming Bacteria - classification ; Gram-Positive Endospore-Forming Bacteria - genetics ; Gram-Positive Endospore-Forming Bacteria - isolation & purification ; Gram-Positive Endospore-Forming Bacteria - physiology ; identification ; Microbiology ; Microbiology in the medical area ; Mikrobiologi inom det medicinska området ; Miscellaneous ; Molecular Sequence Data ; phosphatidylglycerol ; Phylogeny ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - analysis ; RNA, Ribosomal, 16S - genetics ; soil ; staphylococcus-aureus ; Systematics ; waste-water ; xylanolytic bacterium
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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  • 7
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2013-04, Vol.63 (Pt 4), p.1342-1349
    Description: A previously undescribed aerobic, non-sporulating bacterium, strain G1A_585(T), was isolated from an oligotrophic freshwater lake in Bavaria, Germany. The rod-shaped cells were Gram-stain-negative and non-motile. Based on 16S rRNA gene sequence similarity, strain G1A_585(T) was a member of the family Sphingomonadaceae and shared 〈95.2 % similarity with type strains of all members of the most closely related genus, Sphingopyxis. Phyogenetically, the isolate shared a root with strains of three marine species, Sphingopyxis flavimaris DSM 16223(T), Sphingopyxis marina DSM 22363(T) and Sphingopyxis litoris DSM 22379(T). The polar lipids of strain G1A_585(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, sphingoglycolipids, three glycolipids and one unknown lipid. Ubiquinone-10 was the dominant quinone (93.1 %) and ubiquinone-9 (6.5 %) was also detected. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 38.2 %); C16 : 1ω7c (33.6 %) and C14 : 0 2-OH (17.8 %). The major polyamine was spermidine and traces of 1,3-diaminopropane, putrescine and spermine were also detected. The DNA G+C content of strain G1A_585(T) was 55.7 mol% and the isolate was oxidase- and catalase-positive. Based on the phylogenetic relationship, the low DNA G+C content compared with most other members of the genus Sphingopyxis and the presence of signature nucleotides in the 16S rRNA gene sequence, a novel species in a new genus and species, Sphingorhabdus planktonica gen. nov., sp. nov., is proposed; the type strain of Sphingorhabdus planktonica is G1A_585(T) ( = DSM 25081(T)  = LMG 26646(T)). Because Sphingopyxis flavimaris DSM 16223(T), Sphingopyxis marina DSM 22363(T) and Sphingopyxis litoris DSM 22379(T) form a phylogenetic group together with strain G1A_585(T) that is clearly separated from all other known Sphingopyxis strains and share signature nucleotides, these three Sphingopyxis strains are reclassified as members of the proposed novel genus Sphingorhabdus: Sphingorhabdus flavimaris comb. nov. (type strain SW-151(T) = DSM 16223(T) = KCTC 12232(T)), Sphingorhabdus marina comb. nov. (type strain FR1087(T) = DSM 22363(T) = IMSNU 14132(T) = KCTC 12763(T) = JCM 14161(T)) and Sphingorhabdus litoris comb. nov. (type strain FR1093(T) = DSM 22379(T) = IMSNU 14133(T) = KCTC 12764(T) = JCM 14162(T)).
    Subject(s): Bacterial Typing Techniques ; Base Composition ; DNA, Bacterial - genetics ; Fatty Acids - analysis ; Fresh Water - analysis ; Germany ; Lakes - microbiology ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Spermidine - analysis ; Sphingomonadaceae - classification ; Sphingomonadaceae - genetics ; Sphingomonadaceae - isolation & purification ; Ubiquinone - analysis ; Water Microbiology
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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  • 8
    Language: English
    In: International journal of medical microbiology, 2012, Vol.302 (3), p.117-128
    Description: Abstract While flagella-independent motility has long been described in representatives of the genus Acinetobacter , the mechanism of motility remains ambiguous. Acinetobacter baumannii , a nosocomial pathogen appearing increasingly multidrug-resistant, may profit from motility during infection or while persisting in the hospital environment. However, data on the frequency of motility skills among clinical A. baumannii isolates is scarce. We have screened a collection of 83 clinical A. baumannii isolates of different origin and found that, with the exception of one isolate, all were motile on wet surfaces albeit to varying degrees and exhibiting differing morphologies. Screening a collection of transposon mutants of strain ATCC 17978 for motility defects, we identified 2 akinetic mutants carrying transposon insertions in the dat and ddc gene, respectively. These neighbouring genes contribute to synthesis of 1,3-diaminopropane (DAP), a polyamine ubiquitously produced in Acinetobacter . Supplementing semi-solid media with DAP cured the motility defect of both mutants. HPLC analyses confirmed that DAP synthesis was abolished in ddc and dat mutants of different A. baumannii isolates and was re-established after genetic complementation. Both, the dat and ddc mutant of ATCC 17978 were attenuated in the Galleria mellonella caterpillar infection model. Taken together, surface-associated motility is a common trait of clinical A. baumannii isolates that requires DAP and may play a role in its virulence.
    Subject(s): 1,3-Diaminopropane ; Acinetobacter baumannii ; Acinetobacter baumannii - genetics ; Acinetobacter baumannii - isolation & purification ; Acinetobacter baumannii - physiology ; Acinetobacter baylyi ; Acinetobacter Infections - microbiology ; ADP1 ; Animals ; Chromatography, High Pressure Liquid ; Cross infection ; dat ; ddc ; Diamines - metabolism ; DNA Transposable Elements ; Drug resistance in microorganisms ; Galleria mellonella ; Gene Knockout Techniques ; Genetic Complementation Test ; Humans ; Infectious Disease ; Lepidoptera ; Locomotion ; Medical colleges ; Medical Education ; Metabolic Networks and Pathways - genetics ; Motility ; Mutagenesis, Insertional ; Nosocomial infections ; Polyamines ; Transposons ; Virulence ; Virulence Factors - metabolism
    ISSN: 1438-4221
    E-ISSN: 1618-0607
    Source: DOAJ Directory of Open Access Journals - Not for CDI Discovery
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  • 9
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2016-02, Vol.66 (2), p.881-888
    Description: The taxonomic position of five strains isolated from horse faeces, and which shared identical 16S rRNA gene sequences, were studied. Cells of all isolates are Gram-stain-negative, obligately aerobic and have a rod-shaped appearance. The strains show highest 16S rRNA gene sequence similarities to (98.3 %), (98.0 %), (97.9 %) and (97.9 %). Whole-genome sequencing of strain 114 and phylogeny reconstruction based on a core set of 1061 genes indicated that CIP 107468 was the closest relative among species of the genus for which whole genome sequences are available. The genomic DNA G+C content of strain 114 is 34.9 mol%, which is lower than any other value reported for the genus . The predominant polyamine is 1,3-diaminopropane, which is typical for the genus . The most abundant fatty acids are C ω7 and/or iso-C 2-OH (36 %) and C (28 %). The proportion of C ω9 (7 %) is distinctively low compared to most species of the genus. The major ubiquinone of strain 114 is Q-9. Microscopic studies revealed the presence of pili and the absence of flagella. The capability of all five strains to utilize l-arabinose and gentisate as well as their lack of growth at temperatures of 41 °C and above provide sufficient criteria to distinguish the isolates from all species of the genus with validly published names. Based on these combined data, the five isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 114 ( = DSM 27228  = CCUG 65204 ).
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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  • 10
    Language: English
    In: International journal of systematic and evolutionary microbiology, 2016-10, Vol.66 (10), p.4027-4032
    Description: A Gram-stain-negative bacterium was isolated from Lake Michigan water. 16S rRNA gene sequence analysis revealed that strain 1458T had a sequence similarity to Filimonas lacunae YT21T, Sediminibacterium goheungense HME7863T, Parasegetibacter terrae SGM2-10T, Sediminibacterium ginsengisoli DCY13T, Terrimonas ferruginea DSM 30193T, Lacibacter cauensis NJ-8T, Flavihumibacter solisilvae 3-3T, Parasegetibacter luojieneis RHYL-37T, Vibrionimonas magnilacihabitans MU-2T and Parafilimonas terrae 5GHs7-2T with values of 93.4, 92.3, 91.9, 91.9, 91.8, 91.6, 91.6, 91.6, 91.5 and 90.4 %, respectively. The primary cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1G and summed feature 3 (iso-C15 : 0 2-OH/C16 : 1ω7c). The primary polar lipids were phosphatidylethanolamine and an unidentified polar lipid only detectable after total polar lipid staining. The quinone system was menaquinone MK-7, and in the polyamine pattern, sym-homospermidine was predominant. Additional phenotypic characteristics included growth at 15 to 40 °C and pH 5.0 to 8.0, a salt tolerance range of 0 to 2.0 % (w/v), production of orange cell-bound pigment flexirubin, and gliding motility. After phenotypic, chemotaxonomic and molecular analyses, strain 1458T was identified as a novel species of the genus Filimonas, for which the name Filimonas aurantiibacter sp. nov. is proposed. The type strain is 1458T (=NRRL B-65305T=LMG 29039T). An emended description of the genus Filimonas is also provided.
    Subject(s): Bacterial Typing Techniques ; Bacteroidetes - classification ; Bacteroidetes - genetics ; Bacteroidetes - isolation & purification ; Base Composition ; DNA, Bacterial - genetics ; Fatty Acids - chemistry ; Lakes - microbiology ; Michigan ; Phosphatidylethanolamines - chemistry ; Phylogeny ; Pigmentation ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Spermidine - analogs & derivatives ; Spermidine - chemistry ; Vitamin K 2 - analogs & derivatives ; Vitamin K 2 - chemistry
    ISSN: 1466-5026
    E-ISSN: 1466-5034
    Source: Microbiology Society
    Source: Alma/SFX Local Collection
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