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  • 1
    Language: English
    In: Fertility and sterility, 2018-11, Vol.110 (6), p.1109-1117.e2
    Description: To study the impact of in vitro fertilization, with or without intracytoplasmic sperm injection (IVF/ICSI), frozen-embryo transfer (FET), and intrauterine insemination (IUI) on fetal growth kinetics throughout pregnancy and to compare the different modes of conception. Retrospective cohort study. University. A total of 560 singleton pregnancies were included (96 IVF, 210 ICSI, 121 FET, and 133 IUI). None. We compared crown-rump length (CRL) at the first trimester (T1: 11-13 weeks of gestation [WG] + 6 days), estimated fetal weight (EFW) at the second (T2: 21-23 WG + 6 days) and third (T3: 31-33 WG + 6 days) trimesters, and birth weight (BW) z-scores with those in the reference curves (Papageorghiou for T1, and Ego M2 for T2, T3, and birth). Multivariate analyses were performed. For T1, the CRL was longer than the reference curve whatever the assisted reproductive technique (ART). For T2, EFW was significantly greater for all groups compared with the reference curve, and for T3 only FET singletons had a greater EFW. ICSI, IVF, and IUI singletons had a significantly lower BW compared with reference curves. For all ART fetuses, growth kinetics differed from T2. Only FET fetuses maintained their significantly above-reference growth values. The proportion of fetuses for which at least one period of growth loss was observed from T2 to birth was higher after IVF, ICSI, and IUI than after FET. For the first time, we have highlighted that fetal growth kinetics differed from T2 depending on the ART protocols used. They could have an impact on trophoblastic invasiveness and might lead to long-term health effects.
    Subject(s): Pregnancy ; Fetus ; Growth ; Analysis ; Index Medicus ; Life Sciences ; Human health and pathology ; Gynecology and obstetrics
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 2
    Language: English
    In: Andrology (Oxford), 2017-03, Vol.5 (2), p.370-380
    Description: Summary Macrozoospermia is characterized by a high proportion of abnormal spermatozoa with enlarged heads. So far, it has been associated with mutations only in the Aurora Kinase C gene (AURKC) in some cases. Although many publications have reported failure to conceive in couples with macrozoospermia, a few others have described successful pregnancies, thus raising questions as to whether ICSI and AURKC genetic screening should be recommended in all patients with macrozoospermia. First, we report on two monozygotic twins presenting macrozoospermia for whom the genetic status was explored (Aurora Kinase C sequencing) and whole semen and gradient‐selected spermatozoa were analyzed, using Fluorescent In Situ Hybridization (FISH), Electron Microscopy and flow cytometry. Additionally, FISH analysis was performed on individually selected uniflagellate spermatozoa with normal sized heads. Second, we also provide an updated review of patients with macrozoospermia gathering the percentage of enlarged head spermatozoa, the genetic status and pregnancy outcomes. Both twins carried a homozygous mutation of AURKC. Spermocytograms showed means of 86% and 83.5% of enlarged head forms. FISH analyses showed that normal head size, uniflagellate spermatozoa had an aneuploid or polyploid nucleus despite a high level of selection. SEM analysis also showed special intranuclear inclusions in enlarged head spermatozoa. Our data together with cases reported in the literature allowed us to recommend that the AURKC gene should be sequenced when the sperm contains 30% or more of enlarged head spermatozoa, and when a mutation is found, ART should not be performed. Our analyses provide information that could greatly help practitioners in their decision‐making with regard to optimal care of patients with macrozoospermia.
    Subject(s): macrozoospermia ; pregnancy outcomes ; aurora kinase C gene ; assisted reproductive technologies ; Twins - genetics ; Genetic Testing ; Teratozoospermia - genetics ; Humans ; Adult ; Male ; Aurora Kinase C - genetics ; Reproductive Techniques, Assisted ; Sperm Head ; Analysis ; Genetic screening ; Reproductive technology ; Twins ; Mutation ; Index Medicus ; Life Sciences ; Genetics
    ISSN: 2047-2919
    ISSN: 0105-6263
    ISSN: 2047-2927
    E-ISSN: 2047-2927
    E-ISSN: 1365-2605
    Source: Academic Search Ultimate
    Source: Wiley Online Library All Journals
    Source: Alma/SFX Local Collection
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  • 3
    Language: English
    In: Basic and clinical andrology, 2015, Vol.25 (1), p.6-6
    Description: To compare sperm parameters and intracytoplasmic sperm injection (ICSI) outcomes for testicular spermatozoa frozen on the day of the biopsy (DO) with those frozen after 24 h of in vitro culture (D1). In this retrospective study, from 1999 to 2012, forty-nine azoospermic patients were included to compare sperm (motility and viability) and outcomes (fertilization (FR), implantation (IR), pregnancy (PR) and delivery rates (DR)). The in vitro culture increased total motility (+2.8 %, p = 0.0161) but decreased viability (-8.3 %, p = 0.007). After 24 h of culture, the post-thaw changes in motility and viability were not significant. Twenty-six couples underwent ICSI: thirty-four ICSI were performed with spermatozoa cryopreserved at D0 and eighteen with spermatozoa frozen at D1. Cumulated IR and DR were lower for ICSI with D1 spermatozoa than with D0 spermatozoa (IR: 21.6 % with D0 vs. 9.8 % with D1, p = 0.102; DR: 27.5 % with D0 vs. 8.3 % with D1, p = 0.049). Despite improving motility, freezing spermatozoa 24 h after testicular biopsy had a potential negative effect on ICSI outcomes, notably on delivery rates. These results may be related to the detrimental impact of the additional culture on the nuclear integrity of sperm.
    Subject(s): Spermatozoïde testiculaire ; ICSI ; Congélation ; Outcome ; Issues ; Culture ; Freezing ; Testicular sperm
    ISSN: 2051-4190
    ISSN: 1166-2654
    E-ISSN: 2051-4190
    Source: BioMedCentral Open Access
    Source: PubMed Central
    Source: Alma/SFX Local Collection
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  • 4
    Language: English
    In: Cellular and molecular life sciences : CMLS, 2020-02, Vol.77 (3), p.511-529
    Description: The sperm acrosome is a lysosome-related organelle that develops using membrane trafficking from the Golgi apparatus as well as the endolysosomal compartment. How vesicular trafficking is regulated in spermatids to form the acrosome remains to be elucidated. VPS13B, a RAB6-interactor, was recently shown involved in endomembrane trafficking. Here, we report the generation of the first Vps13b-knockout mouse model and show that male mutant mice are infertile due to oligoasthenoteratozoospermia. This phenotype was explained by a failure of Vps13b deficient spermatids to form an acrosome. In wild-type spermatids, immunostaining of Vps13b and Rab6 revealed that they transiently locate to the acrosomal inner membrane. Spermatids lacking Vps13b did not present with the Golgi structure that characterizes wild-type spermatids and showed abnormal targeting of PNA- and Rab6-positive Golgi-derived vesicles to Eea1- and Lamp2-positive structures. Altogether, our results uncover a function of Vps13b in the regulation of the vesicular transport between Golgi apparatus, acrosome, and endolysosome.
    Subject(s): Spermatids - metabolism ; Acrosome - metabolism ; Vesicular Transport Proteins - metabolism ; Male ; Protein Transport - physiology ; Spermatozoa - metabolism ; Mice, Knockout ; Spermatogenesis - physiology ; Animals ; Lysosomes - metabolism ; Golgi Apparatus - metabolism ; Biological Transport - physiology ; Mice ; Analysis ; Biosynthesis ; Index Medicus
    ISSN: 1420-682X
    E-ISSN: 1420-9071
    Source: Alma/SFX Local Collection
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  • 5
    Language: English
    In: Fertility and sterility, 2018-02, Vol.109 (2), p.302-309.e1
    Description: To study whether the closed culture system, as compared with a benchtop incubator with similar culture conditions, has a positive impact on intracytoplasmic sperm injection (ICSI) outcomes. Randomized controlled trial. University hospital. A total of 386 patients undergoing ICSI cycles with at least six mature oocytes were randomized. Of these patients, 195 were assigned to the group with culture in a time-lapse imaging (TLI) system (EmbryoScope) and 191 to the group with culture in the G185 K-System (G185). Rate of implantation (primary endpoint) and embryo morphology grade. No significant differences were found in the implantation rates. The proportion of high-grade embryos on day 2 was significantly higher in the TLI group compared with the G185 group (40.4% vs. 35.2%). The impact of the incubator on embryo morphology remained significant in multivariate analysis, which took into account the woman's age, the rank of attempt, and the smoking status (TLI vs. G185: odds ratio = 1.27; 95% confidence interval, [1.04–1.55]). No difference was found in the mean number of frozen embryos, even though the total proportion of frozen embryos was significantly higher in the TLI group than in the G185 group (29.5% vs. 24.8%). No difference in implantation rate was found between the two incubators for fresh cycles. It remains to be determined whether the observed differences in embryo morphology and the total number of embryos cryopreserved would translate into higher cumulative outcomes with subsequent frozen embryo transfers. NCT02722252.
    Subject(s): implantation ; embryo morphology ; Closed culture system ; embryo culture ; Index Medicus ; Life Sciences ; Genetics ; Human genetics
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 6
    Language: English
    In: Scientific reports, 2018-02-02, Vol.8 (1), p.2202-13
    Description: Progress in assisted reproductive technologies strongly relies on understanding the regulation of the dialogue between oocyte and cumulus cells (CCs). Little is known about the role of long non-coding RNAs (lncRNAs) in the human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that were abundant in metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, and LINC01118) and CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, and VIM-AS1). These data were validated by RT-qPCR analysis using independent oocytes and CC samples. The functions of the identified lncRNAs were then predicted by constructing lncRNA-mRNA co-expression networks. This analysis suggested that MII oocyte lncRNAs could be involved in chromatin remodeling, cell pluripotency and in driving early embryonic development. CC lncRNAs were co-expressed with genes involved in apoptosis and extracellular matrix-related functions. A bioinformatic analysis of RNA-sequencing data to identify CC lncRNAs that are affected by maternal age showed that lncRNAs with age-related altered expression in CCs are essential for oocyte growth. This comprehensive analysis of lncRNAs expressed in human MII oocytes and CCs could provide biomarkers of oocyte quality for the development of non-invasive tests to identify embryos with high developmental potential.
    Subject(s): RNA, Long Noncoding - analysis ; Oocytes - physiology ; Humans ; Metaphase ; Computational Biology ; Gene Expression Profiling ; Reverse Transcriptase Polymerase Chain Reaction ; Real-Time Polymerase Chain Reaction ; Cumulus Cells - physiology ; Data processing ; mRNA ; Gene expression ; Oocytes ; Chromatin remodeling ; Interleukin 6 ; Reproduction ; Embryogenesis ; Extracellular matrix ; Non-coding RNA ; Pluripotency ; Age ; Apoptosis ; Index Medicus ; Life Sciences
    ISSN: 2045-2322
    E-ISSN: 2045-2322
    Source: Nature Open Access
    Source: Academic Search Ultimate
    Source: PubMed Central
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  • 7
    Language: English
    In: Epigenetics, 2012-05-01, Vol.7 (5), p.440-446
    Description: Alterations to DNA methylation have been attributed to in vitro culture and may affect normal embryo development. We chose to analyze DNA methylation reprogramming in the rabbit which, of the species with delayed transcriptional activation of the embryonic genome, allows easy comparisons between in vivo-developed (IVD) and in vitro-cultured (IVC) embryos. In this species, variations in DNA methylation had not previously been quantified, even in IVD embryos. IVD and IVC embryos were recovered at the 2, 4, 8 and 16-cell, morula and blastocyst stages. Immunostaining for 5-methyl-cytidine and normalization of the quantity of methylated DNA vs. the total DNA content were then performed. Our quantitative results evidenced DNA demethylation during pre-implantation development in both IVD and IVC embryos, but with different kinetics. Demethylation occurred earlier in vitro than in vivo between the 2 and 8-cell stages in IVC embryos, reaching its lowest level, while it only started at the 4-cell stage and ended at the 16-cell stage in IVD embryos. We also showed that an absence of serum from the culture medium significantly altered the degree of DNA demethylation. Finally, at the blastocyst stage, ICM was more methylated than the trophectoderm in all cases. Despite a morphological delay observed in in vitro cultured blastocysts, the difference in DNA methylation between ICM and trophectoderm cells appeared at the same time post-fertilization in IVD and IVC embryos, which may reflect another difference in the dynamics of DNA methylation during blastocyst formation. Our data thus clearly establish an effect of embryonic environment on DNA methylation reprogramming during pre-implantation development in a non-rodent species.
    Subject(s): trophectoderm ; rabbit embryos ; DNA Methylation ; in vivo development ; inner cell mass ; in vitro culture ; Binding ; Proteins ; Landes ; Calcium ; Bioscience ; Biology ; Cell ; Cycle ; Cancer ; Organogenesis ; Rabbits ; Species Specificity ; DNA (Cytosine-5-)-Methyltransferase 1 ; Transcriptional Activation ; Blastocyst - metabolism ; Blastocyst - cytology ; Cytidine - analogs & derivatives ; Embryonic Development ; Culture Media - metabolism ; Embryo, Mammalian - metabolism ; DNA (Cytosine-5-)-Methyltransferases - metabolism ; Immunohistochemistry - methods ; DNA (Cytosine-5-)-Methyltransferases - genetics ; Ectoderm - cytology ; Ectoderm - metabolism ; Animals ; Embryo, Mammalian - embryology ; Time Factors ; Embryo, Mammalian - cytology ; Cytidine - metabolism ; Female ; In Vitro Techniques ; Index Medicus ; Life Sciences ; Development Biology ; Reproductive Biology
    ISSN: 1559-2294
    E-ISSN: 1559-2308
    Source: Taylor & Francis Open Access
    Source: Taylor & Francis:Master (3349 titles)
    Source: PubMed Central
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  • 8
    Language: English
    In: Fertility and sterility, 2016, Vol.107 (1), p.97-103.e4
    Description: Objective To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. Design Retrospective study. Setting Hospital. Patient(s) Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November 2012 to December 2014 were included. A total of 2,449 embryos were assessed for multinucleation rates at the two- and four-cell stage, and 608 transferred embryos were studied for ICSI outcomes. Intervention(s) None. Main Outcome Measure(s) Implantation rate (IR) and live birth rate (LBR) according to the number of multinucleated blastomeres at the two-cell stage: none (Without-MNB2cell ), one (MNB1/2cell ), and two (MNB2/2cell ); morphokinetics of MNB2cell embryos. Result(s) Embryos with MNB1/2cell led to lower IR (27.7%) and LBR (22.7%) than embryos Without-MNB2cell (33.4% and 29.8%, respectively). The MNB2/2cell embryos led to significantly lower IR (18.3%) and LBR (13.4%) than embryos Without-MNB2cell . This difference remained significant in multivariate analysis for implantation (odds ratio 0.57; 95% confidence interval 0.34–0.94) and birth (odds ratio 0.46; 95% confidence interval 0.26–0.80), independently of the other significant parameters (women's age, time of two-cell formation, and multinucleation at the four-cell stage). Among implanted MNB2cell , if cleavage into four cells occurred later than 37 hours after insemination, embryos were significantly more likely to lead to birth. Conclusion(s) The presence of multinucleation at the two-cell stage and more specifically in both blastomeres had a significant negative impact on birth potential. Thus, embryo multinucleation at the two-cell stage should be used as an additional noninvasive criterion for embryo selection.
    Subject(s): Internal Medicine ; Obstetrics and Gynecology ; Birth rate ; implantation ; ICSI outcomes ; embryo multinucleation ; time-lapse system ; Multivariate Analysis ; Humans ; Microscopy, Video ; Cleavage Stage, Ovum ; Embryo Transfer - adverse effects ; Fertility ; Female ; Infertility - physiopathology ; Time-Lapse Imaging - methods ; Retrospective Studies ; Odds Ratio ; Infertility - therapy ; Pregnancy Rate ; Live Birth ; Risk Factors ; Logistic Models ; Treatment Outcome ; Blastomeres - cytology ; Chi-Square Distribution ; Embryo Implantation ; Pregnancy ; Infertility - diagnosis ; Embryo, Mammalian - cytology ; Sperm Injections, Intracytoplasmic - adverse effects ; Cell Nucleus ; Embryonic development ; Spermatozoa ; Embryo ; Analysis ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 9
    Language: English
    In: Fertility and sterility, 2017-10, Vol.108 (4), p.650-658.e2
    Description: To study the impact of severe ovarian hyperstimulation syndrome (OHSS) on beta-hCG kinetics and obstetrical and neonatal outcomes. Retrospective single-center case-control study. University tertiary referral center. A total of 77 patients who presented a clinical pregnancy after IVF and had been hospitalized for severe OHSS were included in this study and compared with 231 controls presenting an IVF-induced clinical pregnancy without OHSS and matched for the year of pregnancy and the number of gestational sacs. None. The outcome of pregnancy (miscarriage, medical abortion, or delivery), beta-hCG values, obstetrical, and neonatal outcomes. After multivariate analysis adjusted for parity, tobacco smoking, presence of polycystic ovary syndrome, age, and body mass index, outcomes of pregnancies were not altered by OHSS. However, there was a trend toward a lower early miscarriage rate in the OHSS group (7.8%) than in the control group (16%). Maternal serum beta-hCG values at different time points of the pregnancy and fold changes of beta-hCG values were lower in OHSS than in controls (268 ± 160 vs. 389 ± 215 IU/L at day 16; and 4.8 ± 1.5 vs. 5.4 ± 1.4 fold change between day 16 and day 20). Beta-hCG also correlated negatively with the number of oocytes retrieved. Incidence of gestational diabetes, gestational hypertension, intrauterine growth restriction, premature birth, and low birth weight did not differ between groups. Although early maternal serum beta-hCG kinetics were modified in women after severe OHSS, the outcomes of these pregnancies remained comparable to those of IVF pregnancies without OHSS. According to these data, pregnancies after severe OHSS do not require particular care compared with IVF pregnancies, but differences in beta-hCG levels and kinetics should be taken into account when interpreting these results.
    Subject(s): beta-hCG ; ovarian hyperstimulation syndrome ; obstetrical outcomes ; in vitro fertilization ; Assisted reproductive technologies ; Fertilization in Vitro - adverse effects ; Severity of Illness Index ; Pregnancy Trimester, First - blood ; Ovarian Hyperstimulation Syndrome - complications ; Humans ; Chorionic Gonadotropin, beta Subunit, Human - blood ; Male ; Ovarian Hyperstimulation Syndrome - blood ; Ovarian Hyperstimulation Syndrome - epidemiology ; Case-Control Studies ; Pregnancy Outcome - epidemiology ; Pregnancy ; Fertilization in Vitro - methods ; Adult ; Female ; Retrospective Studies ; Kinetics ; Pregnancy Rate ; Infants (Newborn) ; Glycoproteins ; Chorionic gonadotropin ; Stein-Leventhal syndrome ; Analysis ; Index Medicus
    ISSN: 0015-0282
    E-ISSN: 1556-5653
    Source: Alma/SFX Local Collection
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  • 10
    Language: English
    In: PloS one, 2016, Vol.11 (3), p.e0150857-e0150857
    Description: In animal studies, extensive data revealed the influence of culture medium on embryonic development, foetal growth and the behaviour of offspring. However, this impact has never been investigated in humans. For the first time, we investigated in depth the effects of embryo culture media on health, growth and development of infants conceived by In Vitro Fertilization until the age of 5 years old. This single-centre cohort study was based on an earlier randomized study. During six months, in vitro fertilization attempts (No. 371) were randomized according to two media (Single Step Medium--SSM group) or Global medium (Global group). This randomized study was stopped prematurely as significantly lower pregnancy and implantation rates were observed in the SSM group. Singletons (No. 73) conceived in the randomized study were included (42 for Global and 31 for SSM). The medical data for gestational, neonatal and early childhood periods were extracted from medical records and parental interviews (256 variables recorded). The developmental profiles of the children in eight domains (social, self-help, gross motor, fine motor, expressive language, language comprehension, letter knowledge and number knowledge--270 items) were compared in relation to the culture medium. The delivery rate was significantly lower in the SSM group than in the Global group (p〈0.05). The culture medium had no significant effect on birthweight, risk of malformation (minor and major), growth and the frequency of medical concerns. However, the children of the Global group were less likely than those of the SSM group to show developmental problems (p = 0.002), irrespective of the different domains. In conclusion, our findings showed that the embryo culture medium may have an impact on further development.
    Subject(s): Fertilization in Vitro ; Culture Media ; Growth ; Humans ; Child, Preschool ; Infant ; Health Status ; Infant, Newborn ; Cohort Studies ; Fertilization in vitro ; Culture media (Biology) ; Embryonic development ; Research ; Child development ; Analysis ; Index Medicus ; Life Sciences ; Human health and pathology ; Gynecology and obstetrics ; Cognitive science ; In vitro fertilization ; Neonates ; Fertilization ; Sperm ; Medical records ; Implantation ; Cardiovascular disease ; Infants ; Gene expression ; Pregnancy ; Embryonic growth stage ; Autism ; Embryogenesis ; Offspring ; Randomization ; Language ; DNA methylation ; Culture media ; Epigenetics ; Birth weight ; Reproductive technologies ; Blood pressure ; Children
    ISSN: 1932-6203
    E-ISSN: 1932-6203
    Source: Academic Search Ultimate
    Source: PubMed Central
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