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  • 1
    Language: German
    In: B.I.T. online, 2013
    Subject(s): Informationsvermittlung (Bibliothekswesen) ; Research Libraries + Technical Libraries (Librarianship) ; Elektronische Bibliotheken (Bibliothekswesen) ; Switzerland (Central Europe). Swiss Confederation ; Schweiz (Mitteleuropa). Schweizerische Eidgenossenschaft ; Wissenschaftliche Bibliotheken + Technische Bibliotheken (Bibliothekswesen) ; Hochschulbibliotheken + Mittelschulbibliotheken (Bibliothekswesen) ; Information Broking (Librarianship) ; Electronic Libraries (Librarianship) ; Library Networks (Librarianship) ; Bibliotheksnetze + Bibliotheksverbünde (Bibliothekswesen) ; University Libraries + College Libraries (Librarianship) ; Library & Information Sciences ; Library & Information Sciences
    Source: Research Collection (ETH Zurich)
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  • 2
    Language: German
    In: 2013
    Subject(s): Informationsvermittlung (Bibliothekswesen) ; Research Libraries + Technical Libraries (Librarianship) ; Elektronische Bibliotheken (Bibliothekswesen) ; Switzerland (Central Europe). Swiss Confederation ; Schweiz (Mitteleuropa). Schweizerische Eidgenossenschaft ; Wissenschaftliche Bibliotheken + Technische Bibliotheken (Bibliothekswesen) ; Hochschulbibliotheken + Mittelschulbibliotheken (Bibliothekswesen) ; Information Broking (Librarianship) ; Electronic Libraries (Librarianship) ; Library Networks (Librarianship) ; Bibliotheksnetze + Bibliotheksverbünde (Bibliothekswesen) ; University Libraries + College Libraries (Librarianship) ; Library & Information Sciences ; Library & Information Sciences
    Source: Research Collection (ETH Zurich)
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  • 3
    Language: German
    Subject(s): Elektronische Bibliotheken (Bibliothekswesen) ; Wissenschaftliche Bibliotheken + Technische Bibliotheken (Bibliothekswesen) ; Hochschulbibliotheken + Mittelschulbibliotheken (Bibliothekswesen) ; Bibliotheksnetze + Bibliotheksverbünde (Bibliothekswesen) ; Informationsvermittlung (Bibliothekswesen) ; Schweiz (Mitteleuropa). Schweizerische Eidgenossenschaft ; Electronic Libraries (Librarianship) ; Research Libraries + Technical Libraries (Librarianship) ; University Libraries + College Libraries (Librarianship) ; Library Networks (Librarianship) ; Information Broking (Librarianship) ; Switzerland (Central Europe). Swiss Confederation ; Library & Information Sciences
    Source: Research Collection (ETH Zurich)
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  • 4
    Language: German
    In: 2015
    Subject(s): Specialist Journals + Scientific Journals (Press) ; Switzerland (Central Europe). Swiss Confederation ; Schweiz (Mitteleuropa). Schweizerische Eidgenossenschaft ; OPEN Access (Scientific Publishing) ; Fachzeitschriften + Wissenschaftliche Zeitschriften (Zeitungswesen) ; OPEN Access (Wissenschaftliches Publizieren) ; Bibliothekswesen + Bibliothekswissenschaften + Bibliotheken ; Librarianship + Library Sciences + Libraries ; Library & Information Sciences
    Source: Research Collection (ETH Zurich)
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  • 5
    Language: English
    In: Plant physiology, November 2013, Vol.163(3), pp.1179-90
    Description: Nodule formation induced by nitrogen-fixing rhizobia depends on bacterial nodulation factors (NFs), modified chitin oligosaccharides with a fatty acid moiety. Certain NFs can be cleaved and inactivated by plant chitinases. However, the most abundant NF of Sinorhizobium meliloti, an O-acetylated and sulfated tetramer, is resistant to hydrolysis by all plant chitinases tested so far. Nevertheless, this NF is rapidly degraded in the host rhizosphere. Here, we identify and characterize MtNFH1 (for Medicago truncatula Nod factor hydrolase 1), a legume enzyme structurally related to defense-related class V chitinases (glycoside hydrolase family 18). MtNFH1 lacks chitinase activity but efficiently hydrolyzes all tested NFs of S. meliloti. The enzyme shows a high cleavage preference, releasing exclusively lipodisaccharides from NFs. Substrate specificity and kinetic properties of MtNFH1 were compared with those of class V chitinases from Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana...
    Subject(s): Signal Transduction ; Hydrolases -- Metabolism ; Medicago Truncatula -- Enzymology ; Plant Proteins -- Metabolism ; Root Nodules, Plant -- Enzymology ; Sinorhizobium Meliloti -- Metabolism
    ISSN: 0032-0889
    E-ISSN: 1532-2548
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  • 6
    In: New Phytologist, August 2005, Vol.167(2), pp.557-570
    Description: •  Expression of Mtchit 3‐3, a class III chitinase gene, is specifically induced by arbuscular mycorrhizal (AM) fungi in roots of the model legume Medicago truncatula and its transcripts accumulate in cells containing arbuscules. •  Agrobacterium rhizogenes‐transformed roots and root‐organ cultures of M. truncatula were used to study effects of Mtchit 3‐3 on AM fungi. •  This work provides evidence for enzymatic activity of the Mtchit 3‐3 gene product and shows with promoter:gus fusions that a 2 kb fragment located 5′ upstream from the translational start codon of Mtchit 3‐3 is sufficient to confer arbuscule‐dependent gene expression. By fusing the Mtchit 3‐3 coding region to the CaMV 35S promoter the expression pattern was disrupted. Surprisingly, disruption stimulated spore germination of Glomus intraradices and Glomus constrictum, and in the case of G. intraradices resulted in a higher probability of root colonization and spore formation. However, no effect on the abundance of arbuscules within colonized roots became apparent. •  These observations demonstrate that disruption of the tight arbuscule‐dependent expression pattern of Mtchit 3‐3 has effects on the early interaction between roots and AM fungi.
    Subject(s): Arbuscular Mycorrhiza ; Class Iii Chitinase ; Medicago Truncatula ; Glomus Constrictum ; Glomus Intraradices ; Root‐Organ Culture ; Spore Germination
    ISSN: 0028-646X
    E-ISSN: 1469-8137
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  • 7
    Language: English
    In: Planta, 2004, Vol.219(4), pp.626-638
    Description: The Medicago truncatula (Gaertn.) ecotypes Jemalong A17 and R108-1 differ in Sinorhizobium meliloti -induced chitinase gene expression. The pathogen-inducible class IV chitinase gene, Mtchit 4 , was strongly induced during nodule formation of the ecotype Jemalong A17 with the S. meliloti wild-type strain 1021. In the ecotype R108-1, the S. meliloti wild types Sm1021 and Sm41 did not induce Mtchit 4 expression. On the other hand, expression of the putative class V chitinase gene, Mtchit 5 , was found in roots of M. truncatula cv. R108-1 nodulated with either of the rhizobial strains. Mtchit 5 expression was specific for interactions with rhizobia. It was not induced in response to fungal pathogen attack, and not induced in roots colonized with arbuscular mycorrhizal (AM) fungi. Elevated Mtchit 5 gene expression was first detectable in roots forming nodule primordia. In contrast to Mtchit 4 , expression of Mtchit 5 was stimulated by purified Nod factors. Conversely, Mtchit 4 expression was strongly elevated in nodules formed with the K-antigen-deficient mutant PP699. Expression levels of Mtchit 5 were similarly increased in nodules formed with PP699 and its parental wild-type strain Sm41. Phylogenetic analysis of the deduced amino acid sequences of Mtchit 5 (calculated molecular weight = 41,810 Da, isoelectric point pH 7.7) and Mtchit 4 (calculated molecular weight 30,527 Da, isoelectric point pH 4.9) revealed that the putative Mtchit 5 chitinase forms a separate clade within class V chitinases of plants, whereas the Mtchit 4 chitinase clusters with pathogen-induced class IV chitinases from other plants. These findings demonstrate that: (i) Rhizobium -induced chitinase gene expression in M. truncatula occurs in a plant ecotype-specific manner, (ii) Mtchit 5 is a putative chitinase gene that is specifically induced by rhizobia, and (iii) rhizobia-specific and defence-related chitinase genes are differentially influenced by rhizobial Nod factors and K antigens.
    Subject(s): Class IV and V chitinase ; K antigen ; ecotypes R108-1 and Jemalong A17 ; Nod factors ; Sinorhizobium ; Suppression of plant defence
    ISSN: 0032-0935
    E-ISSN: 1432-2048
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  • 8
    Language: English
    In: Planta, 2008, Vol.227(3), pp.671-680
    Description: Different arbuscular mycorrhizal fungi (AMF) alter growth and nutrition of a given plant differently. Plant gene expression patterns in response to fungal colonization show a certain overlap when colonized by fungi of the Glomeraceae. However, little is known of plant responses to fungi of different fungal taxa, e.g. the Gigasporaceae. We therefore compared the impact of colonization by three taxonomically different AMF species ( Glomus intraradices , Glomus mosseae and Scutellospora castanea ) on Medicago truncatula at the physiological and transcriptional level using quantitative-PCR. Each AMF developed a species-typical colonization pattern, with a colonization degree of 60% for G. intraradices and 30% for G. mosseae. Both species developed appressoria, intraradical hyphae, arbuscules and vesicles. S. castanea showed a colonization degree of 10% and developed appressoria, intraradical hyphae, arbuscules and arbusculate coils. All AMF enhanced the plant biomass accumulation and nutritional status although not in correlation with the colonization degree. The expression of 10 mycorrhiza-specific or mycorrhiza-associated plant genes could be separated into two clusters. The first cluster, containing arbuscule-induced genes, was highly induced in interactions with G. intraradices and G. mosseae but also slightly induced by S. castanea . The second cluster of genes contained genes that were induced primarily by S. castanea. In conclusion, genes that respond to colonization by fungi of the genus Glomus also respond to Scutellospora . However, there is also a group of genes that is significantly induced only by Scutellospora and not by Glomus species in this study. Our data indicate that genes may be differentially regulated in response to the different AM fungi.
    Subject(s): Arbuscule-induced gene expression ; Glomus ; Medicago truncatula ; Quantitative PCR ; Scutellospora ; Symbiosis-induced
    ISSN: 0032-0935
    E-ISSN: 1432-2048
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  • 9
    Language: German
    In: Churer Schriften zur Informationswissenschaft. Arbeitsbereich Informationswissenschaft
    Subject(s): Hochschulbibliotheken + Mittelschulbibliotheken (Bibliothekswesen) ; Wissenschaftliche Bibliotheken + Technische Bibliotheken (Bibliothekswesen) ; Bibliothekare (Bibliothekswesen) ; Informations- Und Literaturrecherchen (Wissenschaftliche Arbeitstechnik) ; Informationsvermittlung (Bibliothekswesen) ; Benutzerschulung (Bibliothekswesen) ; Modelle + Modelluntersuchung ; Befragung + Fragebogen + Interview (Sprachwissenschaft) ; Bewertung (Allgemein) ; University Libraries + College Libraries (Librarianship) ; Research Libraries + Technical Libraries (Librarianship) ; Librarians (Librarianship) ; Information And Literature Research (Scientific Working Technique) ; Information Broking (Librarianship) ; User Instruction (Librarianship) ; Models + Model Analysis ; Questionnaire + Interview (Linguistics) ; Evaluation (General) ; Library & Information Sciences
    Source: Research Collection (ETH Zurich)
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